The Effect And The Significance Of Nuclear Factor Activator Protein-2 Alpha On Vascular Endothelial Cell Growth Factor Gene/protein Expression Of Human Lung Adenocarcinoma Cancer Cell Line A549

Objective: To observe the effect of AP-2αon VEGF gene/proteinexpression in lung cancer (A549) cell line and the change of this effectafter DDP intervention. To further investigate the role and mechanism ofAP-2αin the progression of lung cancer.Methods: 1, Adenovirus-mediated gene transfection was used todeliver the AP-2αcDAN into lung cancer cells(A549) in vitro and A549cells were transduced with the adenovirus-lacZ reporter gene construct(Ad-lacZ) at multiplicities of infection (MOI) of 10, 30, 50 MOI, Thenthe rate of transfection was examined with X-gal staining.2,A549 cells had been transduced for 24 hrs with Ad- AP-2αat 50,100MOI and VEGF gene/protein expression in them were detected byImmunocytochemical technique and RT-PCR.3,A549 cells had been transduced for 24 hrs with Ad- AP-2αat 50,100MOI with DDP(cisplatin 10mg/l), after DDP intervention, and VEGFgene/protein expression were detected by Immunocytochemical techniqueand RT-PCR.Results: 1, A549 cells were transduced with the Ad-LacZ atmultiplicities of infection (MOI) of 10, 30, 50 MOI for 24hrs, thetransfection efficiency was 42.1%(P＜0.05), 76.8%(P＜0.05) and91.3%(P＜0.05). 50MOI Ad-lacz was chosed to continue next studybecause this MOI Adenovirus-mediated gene transfection efficiency was90% ;2,With increasing Ad-AP-2 titers, the expression of VEGF increasedat levels of mRNA and protein detected by Immunocyto- chemicaltechnique and RT-PCR after transferring the Ad- AP-2αconstruct intolung cancer (A549) cells, and such effect of induction showed a concentration-dependent manners(P＜0.05);3,with increasing Ad-AP-2αtiters, the expression of VEGFincreased at levels of mRNA and protein detected by Immunocyto-chemical technique and RT-PCR after DDP intervention and transferringthe Ad- AP-2αconstruct into A549 cells(P＜0.05).Conelusion:1,AP-2αcan be effectively delivered to A549 cells andcan up-regulate VEGF gene/protein expression, such results suggest thatAP-2αmight promote tumor growth roles in the pathogenesis andprogression of lung cancer;2,DDP intervention can not prevent AP-2αfrom up-regulatingVEGF gene/protein expression, which suggests AP-2αmight influencechemosensitivity during the treatment of lung cancer with DDP.