Effects Of RuanGan ChongJi(RGCJ) Preventing Liver Fibrosis And The Adjustment Of TGF-β1 In Rats With Experimental Liver Fibrosis

Objection To study the treatment effect of RGCJ, a compound, on rats withimmunologic injured liver fibrosis and the possible mechanism.Methods 115 SD rats were randomly divided into five groups: the normal, the model,the colchicines, and the big, middle, small volume of RGCJ. Rats with immunologic injuredliver fibrosis were induced by intraperitoneal injecting activation blood-serum of pig for tenweeks. The colchicines and the RGCJ groups were intragastric administrate from the fifthweek with the colchicines 0.1mg/kg qd. and the RGCJ 7.5g/kg, 5.5g/kg, 3.5g/kg for the big,the middle and the small volume group until the end of the tenth week. At the end of thetenth week, the colchicines and the RGCJ groups remained five rats for each group toobserve the histological form after five weeks, and the other rats were all executed fordetecting the indexes.Results The severity of inflammation and liver fibrosis scorings in rats of the liverfibrotic modle group were obviously higher than those of the normal group (P＜0.01); afterthe rats had been treated by RGCJ and colchicine, the severity of inflammation and liverfibrosis scorings in rats of all the treatment groups became obviously lower than those ofthe liver fibrotic modle group, which showed significant (P＜0.05, P＜0.01); the severity ofinflammation scoring in rats of the big volume of RGCJ group was higher than those of themiddle, small volume of RGCJ group and the colchicines group, which showed significant(P＜0.05, P＜0.01), but the liver fibrosis scoring of all the treatment groups showed nosignificance(P＞0.05). The level of the TGF-β1,and the expression of TGF-β1 in livertissues of the liver fibrotic model group was significantly higher than that of the normalgroup (P＜0.01, P＜0.01); The level of the TGF-β1,and the expression of TGF-β1 of thecolchicines and the RGCJ groups were significant lower than the liver fibrotic modelgroup(P＜0.01, P＜0.01), and the effect of the RGCJ group were better than the colchicinessignificantly(P＜0.01, P＜0.01).Conclusions①RGCJ can relieve the severity of inflammation and liver fibrosis inimmunologic injured rat liver tissues.②TGF-β1 mRNA in liver tissue of liver-fibrotic rats canexpress increasedly, which showed that TGF-β1 related with the happening and developingof liver fibrosis closely.③Decreasing the expression of TGF-β1, which might be amechanism of the RGCJ anti-liver-fobrotic action.