| The royal jelly includes the many kinds of active material, the ingredient is quite complex. The bee royal jelly is one traditional nutriment and the drugs, can enhance the organism the immunity, anti- oxidizes and delays theorganism to be senile and so on.Along with the age growth, the person produces more free radicals, the free radical causes one of organism senile main reasons, simultaneously, the free radical also can cause the gene function change, thus causes the many kinds of diseases,as the careinogen must be transformed the free radical in order to play the role. This article in order to thoroughly discusses the bee royal jelly the antisenile function factor, separately used the pyrogallic acid method and the Fenton method that has determined Butylated Hydroxytoluene which in the bee royal jellyexisted, to ultraoxygen and hydroxy free radical elimination percentage; Used the highly effective liquid chromatography to determine in the royal jelly the Butylated Hydroxytoluene content; Also through in vitro animal modelexperiment, has determined Butylated Hydroxytoluene to the mouse blood in total SOD, the GSH peroxide enzyme activity influence and to the liver of the MDA content influence. Its each experimental result as follows:Through to the Butylated Hydroxytoluene in the bee royal jelly with highly effective liquidchromatography quantitative analysis, the result demonstrates that royal jelly Butylated Hydroxytoluene content is 12ug/kg.Butylated Hydroxytoluene has the good scavenging action to the ultra oxygen free radicaland the hydroxy free radical. When the Butylated Hydroxytoluene density achieved to 600mg/L, elimination percentage reaches 75.86% to the ultraoxygen, which is strongest; When the Butylated Hydroxytoluene density achieved to 500mg/L, elimination percentage achieves 84.47% to the hydroxy free radical, which is strongest.Comparing with the blank comparison group mouse, Butylated Hydroxytoluene, low, middle in the monitoring group liver of the MDA content are remarkable drop.Comparing with the blank comparison group mouse, Butylated Hydroxytoluene, royal jelly, middle in monitoring group, blood SOD content and active are remarkable rise.Comparing with the blank comparison group mouse, Butylated Hydroxytoluene, low, middle, high group, GSH-PX content and the active are rise. |
