Regulating Study On The Melanogenesis And Its Relationship With White Hair And Pigment Diseases

Along with the development of the society civilization, the people pursue the on--eself increasingly healthy and perfect, the skin that owns the healthy beautiful whiteskin is many persons to long for day and night of, but the chloasma is to affect the m--ost familiar reason of facial appearance, chloasma is occurrence in the hazel of theface go to deep brown, the boundary know of the spot slice, is from various functionwith comprehensive factor with the result that of a kind of difficult pigment diseasethat cure, become perplex the public again hard resolve of problem.At the medical aesthetics and cosmetology, the scholars always at carry on thepigment disease's investigate, pass the experiment, people detection for count not afew chemistry materials to pass the different mechanism influence to wear the livingcreature activity of the melanocyte. However, in these definite valid materials, a lot ofis generally accepted and strong cancer-causing, have of have the obviously poisonousside effect, its safety can't guarantee.Look for a kind of can affect the melanocyte acti-vity and synthesize the gene to carry on to the melanin obviously adjust to control, theagain safe application is in the human body of medicine, is the research purpose ofthis topic.This topic is a part within the natural science fund item of Tienjin " Regulatingstudy on melanogenesis and its relationship with white hair and pigment disease"topic. Expected the foundation research before to have already carried on the develop-ment(melanocyte lump cell of small rat B16F10 and normal melanocyte of person) tothe different and melanocyte, study in the portein level some adjust to control the mat-erial that the melanocyte activates the gene to the melanocyte propagate, the live andmelanin of Tyrosinase influence, and as to it's the function carries on the comparison,examining these medicines to express to Tyrosinase in the gene level subsequently ofadjust and control function, the purpose look for some adjust to control themelanocyteactivates the gene of material, inquiry into its possible function mechanism, at this foundation goes forward to go the first step of clinical trial, cure to do a new quest forthe clinical medicine of the pigment disease.Expected the foundation research before, joined the 75 mg retinoicacid and the27.5 mg hydroquinone respectively to the 5 mls, prepare original aqua density is:50000μmol/L.Add the medicine to go to eventually density, distinguish for: 50, 100,200, 500, 1000, 5000 and 10000μmol/Ls(two density homology).Study to discover,retinoicacid to adjust the live melanin content of tyrosinase under the higher densitycan, still promoting the function that melanocyte propagate at the same time. Retino-icacid and can make the tyrosinase mRNA expression descend, enlarging along withretinoicacid function density or function the extension of time, the tyrosinase mRNAthe expression dies down gradually.The 500μmol/Ls of retinoicacid are itsmost suitable function densities.When retinoicacid with the hydroquinone density allabove 500μmol/Ls, retinoicacid of the same density and can be in conjunctionwith each other with the hydroquinone density, strengthen each other under adjust thetyrosinase function of the melanin content, and above 100μmol/Ls retinoicacidand can also ease the hydroquinone to propagate to the cell of repress the function,come to a to protect the cell to do not need to be result by the injury.Study accordingto the foundation, go out the density of use the medicine to distinguish to 0.015％,0.03％,0.15％,0.3％towards should retinoicacid and the hydroquinone at 500, 1000,5000 and 10000 density ofμmol/Ls, compute, owing to in the clinical application0.15％,0.3％density with retinoicacid is too high, the stimulative compare greatly,unwell in the application face, so sieve 0.015％,0.03％retinoicacid and the hydroquin-one frost, thus, since reserve two be in conjunction with each other, strengthen eachother under adjust the tyrosinase function of the melanin content, retinoicacid to easethe hydroquinone to propagate to the cell of repress the function, come to a to protectthe cell to do not need to be result by the injury, and then reduce the incitement of themedicine, lowering the bad reaction.In addition,retinoicacid can urge the epidermiscell renewal, helping the hydroquinone to wear deeply epidermis.Thetopical cortico- steroid represses the melanin to synthesize through a metabolism activity of reducethe epidermis cell, curing the partial product retinoicacid, the hydroquinone etc. theconsociation application, not only take effect early, and can repress the MC to synthe-size and secrete effectively, lower the melanin, but also can prevent from retinoica-cid and the incitement of the hydroquinone. For the sake of utmost ease the for exter-nal application side effect of hormone, check the cultural heritage to combine to expe-riment continuously, grope for to fill lower density 0.01％dexamethasone.According to the above research, combine the clinic, to should 500μmol/Ls,prepare an experiments the medicine A(0.015％retinoicacid+0.015％hydroquinone+0.01％dexametha-sone), to should in the density of 1000μmol/Ls, prepare anexperiments the medicine B(0.03％retinoicacid+0.03％hydrquinone+0.01％dexam-ethasone), the conduct and actions experiment medicine, clinical quest that carry onthe chloasma to use the medicine treatment outside.Dispeling clinic last generallyaccepted for accurate and 20％azelaic acid, 3％hydroquinone frost originally theproduct of the hospital) conduct and actions checks against the medicine.In the experience formulation, the density of the hydroquinone is between 2％～5％, far higher than this 0.015％～0.03％of medicine density that study;The retino-Icacid is between 0.025％～0.1％, also higher than use 0.015％～0.03％of medicinein our research.The experience formulation has already used for the clinic with longtimes, the safety that human body experiment has already got the affirmation.Becauseour on trial use the far away below traditional outside of the medicine density to useretinoicacid with the density of the hydroquinone, is very safe doubtless on the clinic,this is also the theories foundation that we didn'ts carry on the animal to experiment.But for being responsible for towards experimenting the patient, each experiment themedicine and must carry on patch test before experiment to experiment, feminine gen-der result of chloasma patient then can enter experiment.Design to is divided into the male, female(have something to do with gestation)to thechloasma patient, female(have nothing to do with gestation)3 groups,120 each group.Know what happened to agree the book and match into a conditional tetreceiv-er towards sign, the random single and blind method of male 120 patient adoptions ofset uses the medicine A, medicine B,20％azelaic acid, 3％hydroquinone, a medicineis each to observe 30. Female (gestation relevant) with a set with together male meth-od of a cent of female(the gestation is irrelevant).The medical history all is come inthe patient among 2004.1-2006.1 out-patient services of my hospitals seek medicaladvice.Actual 348 of observation patient.(have 1 because of the face the pain withd-raws the treatment obviously,11 yellow chloasma patients shed off)After washing face sooner or later everyday draw a layer observation medicinein the part of the chloasma with thin, use the medicine the period avoids the insolatio-n.Observe 4 weeks continuously, after first time and treatments 1,2,4 weeks are eachwith visit once, at 4 weekend judgment curative effects.4 cases all did not see the whole body bad to respond in cure process, the A med-icine contain one person take place the bad reaction, the B medicine contain 4 people,the main performance is a face light the erythema of the degree, take off the scraps toall have not yet the processing, with alleviate with the extension nature of the medici-ne time.The 20％azelaic acid respond 18 badly,3％hydroquinone frost responds 13badly.This study bad to respond the main performance at use the pharmacy of an eryt-hema of a skin, take off the scraps or the pains, in addition to 20％azelaic acid have 1people because of the pain obviously but withdraw treatment, remaining all did not dothe processing, along with use the medicine time extension the nature alleviate.We discover in clinic study, although the chloasma patient of the male, female(have something to do with gestation), female(have nothing to do with gestation)may have the dissimilarity in being taken bad the mechanism, three use the medicinetreatment face curative effect of thechloasma outside use(retinoicacid and hydroqu-inone) up have no obvious difference.Then the outside have no obvious differencewith 3 efficient and bad reaction rates of male, female(have something to do withgestation), female(have nothing to do with gestation) of the A medicine;Medicine B,20％azelaic acid,and 3％hydroquinone are similia with medicine A.Past for thesake of the research result more accuracy, we are the male, female(have something todo with gestation), female(have nothing to do with gestation)3 combinations combine,and carry on statistics to learn the analysis to the data after merge, A medicine, B,20％azelaic acid,3％hydroquinone of 4 kinds of medicine treatments chloaSma efficientdistinguish to 78.16％,94.38％,81.60％,81.18％,4 kinds of medicine curative effectdifference statisticses to learn the meaning(P＜0.05), a medicine the curative effect isis obvious and high in A,20％azelaic acid and 3％hydroquinone, elucidation B medi-cine the curative effect is best.The bad reaction rate distinguishes to 1.15％, 4.49％,20.68％,15.29％, the bad reaction rate difference statisticses to learn the meaning(P＜0.05), the bad reaction rate of A medicine, B medicine is obvious low in 20％azelaic acid and 3％hydroquinone.In this research, we put forward the density very low treatment retinoicacid ofthe chloasma for the first time and the hydroquinone formulation, and discover that itcures the result higher cure reasult than the method in the tradition, the safety ishigh.Because B medicine in retinoicacid is far with the density of the hydroquinonefar small use the density of the medicine in the traditional outside, past stimulativevery small, bad the reaction is low, but because of two be in conjunction with thefunction to the melanocyte, having the good treatment result for the chloasma, is anopposite comparison the safety cures the valid product of the chloasma, there is goodand applied foreground.