The Influence Of Expression Activity Of Vascular Growth Factor PDECGF/TP And VEGF In Interstitial Cystitis Patients

Objective: Interstitial cystitis(IC) often displays frequent micturition, urgent micturition, Suprapubic region and cavitas pelvis pain after bladder engorge and pain relieve after urination. At present, there is short of specific diagnosis standard about it. Doctor Hunner made the initial definition of interstitial cystitis in 1915, but it's not received until NIDDK formulated diagnostic criteria of interstitial cystitis. But people generally regarded it as a strict and inconvenience criteria to clinical application. Consequently, people constantly look for new diagnostic methods, but it still lack consummate diagnositic and antidiastole criteria. Because of the homoplastic clinical situation and possible homoplastic pathologic diagnosis between CP /CPPS and IC, some research workers considered that soem CP /CPPS patients featured with chronic pelvic floor pain and urinary passage symptom had high possibility to be IC. The diagnostic criteria of IC made by NIDDK included Hunner ulcer or mucosal bleeding point. But the mechanisms for mucosal bleeding point remain unclear. Therefore, we investigated the relationship between the cystoscopic findings of vascular events and the expression of angiogenic growth factors in bladders in IC to identify the possible mechanisms inducing glomerulations in IC.Methods: We screen patients who had clinical symptom of frequent micturition, urgent micturition, Suprapubic region and cavitas pelvis pain after bladder engorge and pain relieve after urination. Then, we made urine routine, bacterial culture and urine acid-fast bacilli to remove unsuitable patients. 12 patients during hydrodistention using spinal anesthesia were identified with NIDDK criteria and 12 asymptomatic cases. Bladder biopsies were performed in those cases. we used Routine Pathologic Examination (H.E) to observe mast cell and immunohistochemical staining for platelet deriver endothelial cell growth factor/thymidine phosphorylase(PDECGF/TP) and vascular endothelial growth factor(VEGF) was also performed. Afterwards, evaluate those sample with immunohi-stochemical scores(IHS).Results: The 12 patients had glomerulations during cystoscopic examination, the blood flow in bladder wall vessels. using Routine Pathologic Examination (H.E) , we observed more mast cell in IC compared to an control group(P<0.05). In patients with glomerulations a high positive rate for PDECGF/TP and VEGF staining was observed, compared to those in asymptomatic patients(p<0.05).Conclusion: IC patients has much more mast cell than asymptomatic patients, in asymptomatic patients has a very few mast cell. Glomerulations during hydrodistention are highly associated with the over expression of angiogenic growth factors(PDECGF/TP and VEGF) in the bladder. Thus, it seems that neovascularization promoted by angiogenic growth factors has an important role in the pathogenesis of IC, inducing glomerulations during hydrodistention. PDECGF/TP and VEGF play an important role in pathogeny of new vessels formation, through detecting PDECGF/TP and VEGF antibody we can easily screen or finally diagnose IC, thus supply theory instruction to clinical diagnosis of IC.