C-teminal Telopeptides Of Type Ⅰ Collagen In Gingival Crevicular Fluid And Periodontitis

Objectives: The aim of the study was to examine the C-teminal telopeptides of typeⅠcollagen(CTX) in gingival crevicular fluid(GCF) and to introduce a reliable diagnostic method available to detect active periodontal disease before the tissue destruction is evident, and in further to explore the relationship between the level of degradative CTX and that of level trypsin-like enzyme produced by periodontopathogenic key bacteria.Methods:1 Sixty individuals were enrolled in the study. gingival crevicular fluid and corresponding samples of subgingival plaque were collected and pooled after the supragingival plaque removed. 77 gingival crevicular fluid and 77 subgingival plague samples were collected.2 Clinical parameters: Plaque index, gingival index, the greatest probing depth , attachment level, tooth mobility in each experimental tooth were examined and recorded.3 CTX levels in GCF were quantified using Enzyme-linked immunosorbent assay (ELISA). 4 BANA experiment: 100ul subgingival plague sample or bacteria solution was added into BANA solution (1mM 100ul), and incubated overnight at 37℃. The color was developed by addition of 0.25% Fast Black K and hydrolyzed at 37℃for 5min. At last the solution was measured with ELISA instrument reader at 450nm.Results:1 The mean CTX concentration of healthy persons in GCF was 140.17±32.94nmol/L.2 The mean CTX concentration of periodontal disease patients in GCF was 362.07±165.26 nmol/L.3 CTX concentration of active periodontitis patients in GCF: median was 522.21 nmol/L, 95% reference ranges was 382.78-786.41nmol/L. CTX concentration of non-active periodontitis patients in GCF: median was 250.29 nmol/L, 95% reference ranges was 180.24-354.58nmol/L.4 The level of CTX of healthy persons and periodontitis patients: the Mann-Whitney Test was performed, and showed there was a statistical significance between the two groups(P<0.05).5 The level of CTX of active periodontitis patients and non-active periodontitis patients: the Mann-Whitney Test was performed, and showed there was a statistical significance between the two groups(P<0.05).6 The relationship between CTX concentration in GCF and clinical indexes:Using Kruskal-Wallis test, a statistical significance was found in CTX concentration among different plague indexes, gingival indexes, the greatest probing depths, attachment levels. Modest correlations were found between GCF CTX concentration and plague index(r=0.386), gingival index(r=0.366), the greatest probing depth(r=0.382), attachment level(r=0.314). However, there was no statistically significant differences between GCF CTX concentration and tooth mobility(P>0.05).7 The relationship between GCF CTX and trypsin-like enzyme was analyzed with Spearman test, there was a positive correlation r=0.468,P<0.05.Conclusions:1 The mean CTX concentration of healthy persons in GCF was 140.17±32.94nmol/L.The mean CTX concentration of periodontal disease patients in GCF was 362.07±165.26 nmol/L .CTX concentration of active periodontitis patients in GCF: 95% reference ranges was 382.78-786.41nmol/L. CTX concentration of non-active periodontitis patients in GCF: 95% reference ranges was 180.24-354.58 nmol/L.2 The mean CTX concentration of periodontitis patients was significantly higher than that of healthy individuals. It is possible that high GCF CTX concentrations are related to active periodontal tissue destruction. There is high value for in GCF CTX diagnosis of periodontitis and prediction future disease progression.3 There were positive correlations between GCF CTX concentration and clinical parameters, but values of r2 are low. Traditional clinical parameters are static diagnostic parameters, indicating disease history and not current disease status, however, the level of CTX is probably to give information about tissue destruction process beyond the reach of the clinical parameters.4 There was positive statistically correlations between GCF CTX concentration and optical density value of liquid BANA test. It is shown that trypsin-like enzyme could be involved in the process of the degradationⅠcollagen .