| Objective to find out a effective isolated technique and cryopreservedsolution to improve the viability of hepatocyte through this research.Method using weight 20-30g Kunming mouse as provider.establishan improved collagen perfusion technique to isolate the mousehepatocyte .then cryopreserve the isolated mouse hepatocyte with differentcryopreserved solutions,.with usual cryopreserved protocol in nitrogen liquid.And thaw the cryopreserved hepatocytes on half month ,one month ,halfand one month ,two month respectly.see theirs vialbility ,especial functionsand morphology.Result (1) using the improved collangen perfusion technique,we canget a high yiedility of mouse hepatocytes and the vialbility is up to 90％. (2)the vialbility of trial group after thawed on half month ,one month ,half andone month,two month are nearly 80％with both TB and PI exclusion ,thecontrol group are nearly 50％with both TB and PI exclusion, two groups aresignificantly different (P＜0.05) ;the leakage ALT of trial group are nearly13.5,and the control group are nearly 24.0, two groups are significantlydifferent(P＜0.05); the leakage of AST of trial group are nearly 15.0,and theconrol group are nearly 25.0, two groups are significantly different (P＜0.05); the leakage of LDH of the trial group are nearly 15.0,and the controlgroup are nearly 26.0,two groups are significantly different (P＜0.05).thesynthesis of ALB of the trial group are nearly 3.4 ,and the control group arenearly 2.6,two groups are significantly different (P＜0.05) .(3) there are nosignificant differences in two groups of its vialbility, the leakage of ALT, ASTand LDH and synthesis of ALB on half month,one month,half and twomonth ,two month(P＞0.05).Conclusion (1) The trial cryopreserved solution can effectively protectthe hepatocytes from cryopreserved injury in this reserach. (2)mouseheatocytes can cryopreserve in nitrogen liquid for two month with nochanges on terms of vialbility and especial functions with trialcryopreserved solution.
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