Effects Of Lexolic Preconditioning On The TNF-α And Protection In Hepatic Ischemia-reperfusion In Rabbits

Objective: To explore the Effects of Lexolic preconditioning on the TNF-a and protection in hepatic ischemia -reperfusion in rabbitsMethods: 30 rabbits were randomly divided into 3 groups: normal control group (Group A), ischemia-reperfusion 2hours group (Group B) and pretreated the ischemia-reperfusion with Ilexolic group(Group C). 10 rats each group.The ischemia-reperfusion model was produced by Pringle's method. It was produced by claming the hepatic artery and portal vein with a clamp for 30 minutes. then reperfusing 2 hours. The hepatic tissues and blood samples were collected 30 minutes after ischemia and 2 hours after reperfusion in the B,C groups. Then the activity of MDA; the concentration of TNF-α, ALT and AST were measured and hepatic tissue have the pathological examination.RESULTS1. Compared to Group A, the ALT, AST, the activity of MDA, the TNF-αall increased in B, C. And the injury of liver was found by pathological examination.2. Compared to Group B, the medicine of C produced predominanteffective(P<0.05).CONCLUSION ILa could reduce the TNF-α, decreasing the concentration of oxygen free radical. Improving the energy metabolism of the ischemic liver tissue, prevent and reduce the hepatic ischemia reperfusion injury...