Quantitative Expression And Clinical Significance Of Fas And FasL MRNA In Non-Small Cell Lung Cancer

Objective To study the quantitative expression and clinicalsignificance of Fas and FasL mRNA in non-small cell lung cancer(NSCLC).Methods Real-time fluorescent quantitative RT-PCR method Was applied toinvestigate the quantitative expression of Fas and FasL mRNA in NSCLC andnormal lung tissue. Results In NSCLC, The levels of Fas mRNA(t=1.9773, P=0.0540) and FasL mRNA (t=1.5797, P=0.1210) were of no differencebetween squamous carcinoma and adencarcinoma (P＞0.05).The levels of Fas mRNAexpression in NSCLC were significantly lower than those in normal tissues(t'=-5.6634, P＜0.05), and positive expression of Fas mRNA was correlatedwith pathological grades(F=3.81, P=0.0296),clinical stages(F=5.43,P=0.0029)and lymphatic metastasis(t=2.6621, P=0.0107), (P＜0.05);The levelsof FasL mRNA expression in NSCLC were singnificantly higher than those innormal tissues (t'=4.8258, P＜0.05), and positive expression of FasL mRNAwas correlated with pathological grades(F=33.31, P=0.0000),clinicalstages(F=9.82, P=0.0000)and lymphatic metastasis(t'=3.6015, t'(0.0500)=2.0627), (P＜0.05); The positive expression levels of Fas mRNA inNSCLC showed negative correlation with that of differentiation and positivecorrelation with that of lymph node metastasis and clinical stages; And thepositive expression levels of FasL mRNA in NSCLC showed positive correlationwith that of differentiation and positive correlation with that of lymph nodemetastasis and clinical stages. There was no correlation in the expressionsbetween Fas and FasL mRNA (rs=0.2129, P＞0.05). Conclusions Abnormalexpression of Fas and FasL mRNA act correlatively to the originality,development,infiltration,metastasis and malignancy, The expression ofFas and FasL mRNA presents singnificance in evaluation of the malignancy andits theraputic prognosis of NSCLC.