Studies On The Chemical Constituents Of Nidus Vespae

Nidus Vespae is the nest ofPolistes olivaceous (DeGeer), Polistes japonicus Saussure or Para polybiavaria Fabricius which belongs to insects of vespidae. Nidus Vespae widely dist-uributed in China, which is cheap and obtained easily. Nidus Vespae has been used in traditi-onal medicine for a long time, as external drug commonly, and to be taken orally randomly. Nidus Vespae has many pharmacological activity especially on anti-tumour and anti-inflamm-ation aspect. On the basis of the summary of their chemistry and pharmacology, this thesis taken a further study on chemical consituents ofNidus Vespae and research on it's anti-infla-mmation activity.From the methanol extract of the Nidus Vespae, by solvent extraction, repeated column chromatography and recrystallization, 16 compounds were isolated. On the basis of physico-chemical properties and spectroscopic analysis, 13 compounds were identified as: 2β,3α-4-acetyl-7-[(2- acetamide)ethyl]- 3-hydroxy-2-(3,4-dihydroxyphenyl)-2H-benzo[b][1,4] oxazin (1), 8-hydroxyquinoline-4-one(2), thymidine(3), p-dihydroxy benzene (4), 4-hydroxybenzoic acid (5), protocatechuic acid(6), caffeic acid(7), ferulic acid(8), kaempferol (9), rutin(10), phthalic acid isodibutyl ester(11), heptadecane(12)andβ-sitosterol(13).Among them, Compound 1 was a novel compound, Cmpound 2 was a new natural com-pound. Compounds 3-6, 8, 10, 12-13 were isolated from this genus for the first time.This thesis researched the anti-inflammation activity of the n-butanol extractions that got from the methanol extract of the Nidus Vespae by testing mouse's ear swelling.