A Study Of Relation Between Matrix Metalloproteinas E-1 Polymorphism And Coronary Heart Disease In Han Population Of Yunnan.

Objective: To investigate the association between serum level andgene polymorphism of MatrixMetalloproteinase-1(MMP-1) and Coronaryheart disease.Methods :This study was conducted with case-control design in 98patients with angiographically documented Coronary heart disease(CHD) and 102 control subjects who were free from coronary arterydisease. Baseline serum MMP-1 was measured by enzyme-linkedimmunosorbent assay (ELISA) and genotype was determined by polymerasechain reaction-restriction fragment length polymorphism (PCR-RFLP)forthe common-1607 allele function promoter polymorphism of MMP-1 gene .Results:1,No significant difference between CHD and controls wasfound in serum level of MMP-1 ( P>0．05) . 2,The result of individualpolymorphisms analysis showed that the frequency of 2G/2G genotype inpatients(0．41) was significantly lower than that in controlsubjects(0．56) , The frequency of allele-1607 were 0．61 and 0．72 in CHDgroup and control group respectively (p=0．026 ). 3,The frequency of2G/2G genotype of-1607 polymorphism were not statistically differentamong CHD patients with one, two, three or more significantly diseasedvessels (P>0．05 ).Conclusions: 1,Serum level of MMP-1 may not be useful as a predictorof CHD . 2,The present findings suggest that 2G/2G genotype in MMP-1 promoter (-1607) can decrease the susceptibility to CHD in the hanpopulation of Yunnan. 3,The l6O7bp polymorphism may not be usefulas a predictor of the severity of coronary atherosclerosis .