| Background and ObjectivesInvasion and metastasis is the most important factor to influence tumor patient's therapeutic efficacy and prognoses, to control tumor cell's invasion and metastasis is still an important topic in today's tumor research. Research manifest that adhesion molecule has regulation effect to tumor cell's invasion and metastasis, but the specific mechanism unknown. With the study and research of adhesion molecule and some gene product which has function to effect adhesion molecule, these research couldhelp us to understand the mechanism of malignant tumor's invasion and metastasis.Ezrin is one member of the ERM (Ezrin -radixin-moesin) protein family, and has the function of link cell surface receptor especially adhesion molecule and actin cytoskeleton. Ezrin is mainly to present at the top of the cell surface to participate and to keep epithelial cell's polarity. Recent studies have found that Ezrin through regulation of adhesion molecules and signal transduction pathways involved in cell and cell Stromal cells and the interaction between tumor cells may play an important role in the process of invasion and metastasis.FAK is a non-receptor protein tyrosine kinase in cell adhesion lesion. Recent studies have shown that in a variety of tumor tissue have significantly increased expression of FAK, also FAK expression and the biological behavior and the occurrence of certain relevance.E-cadherin is the link between calcium-dependent cell adhesion molecules. Mainly mediated cull adhesion between the same reactions. And maintaining the structural integrity of epithelial cells and the molecular polarity, and play the role of cytoskeleton, the expression of E-cadherin inactivation may lead to decreased cell adhesion, polar disorder, and promote tumor metastasis.The study is testing Ezrin FAK and E-cadherin protein expression in colorectal cancer tissues. Analysis of the relationship between the factors and clinical pathology, and to explore Ezrin Focal adhesion kinase (FAK) and epithelial cadherin (E-cadherin) expression in colorectal cancer tissues and its relation to the relationship between the process of tumor invasion and metastasis.Materials and Methods1. 50 cases of colorectal carcinoma (including 13 well-differentiated adencarcinomas, 37 moderately- or poorly-differentiated adenocarcinomas; 30 cases without lymph node metastasis and 20 with lymph node metastasis) were induced in the study. Samples were taken from tumor site and paracancerous tissues (5cm), which were confirmed pathologically as normal colorectal mucosa. None of the patients had received chemotherapy or radiation therapy before surgery.2. Immunohistochemistry was used to detect the expression of Ezrin, FAK and E-cad in 50 cases of colorectal carcinoma and in 22 cases of normal colorectal mucosa.3. The data was analyzed by SPSS 10.0, x~2-test and Spearman correlation were used to analyze the difference between groups.α=0.05was considered as statistically significant value.ResultsThe expression of Erin was significantly higher in colorectal carcinomas tissue, with moderate or poor differention, lymph node metastasis and Dukes C+D stages than that in the ones with normal colorectal mucosa, high differention, non-metastasis, and Dukes A+B stages (74.00% vs 9.09%, P<0.01;83.78% vs 46.15%, P<0.01; 95.00% vs 60.00%, P< 0.01; 95.00% vs 60.00%, P< 0.01), FAK expression wasmarkedly higher in colorectal carcinomas tissue, lymph node metastasis and with Dukes C+D stages than that in the ones with normal colorectal mucosa, without metastasis and Dukes A+B stages (78.00% vs 45.45%, P<0.01; 100.00% vs 63.33%, P< 0.01; 100.00% vs 63.33%, P< 0.01), but it had no significant correlation with thedifferetion degree of tumors, the sex and ages of patients (P > 0.05). but E-cadexpression was in the contrary situation (36.00% vs 100.00%, P<0.01;24.32% vs 69.23%, P < 0.01; 10.00% vs 53.33%, P < 0.01; 10.00% vs 53.33%, P < 0.01). The expression of Ezrin and E-cad had no marked correlations with the age and sex of patients (P > 0.05). Spearman analysis showed that there existed positive correlation between Ezrin and FAK expression (r_s = 0.346, P < 0.05), and negative correlation between E-cadherin and Ezrin (r_s = -0.410, P < 0.01) as well as E-cadherin and FAK expression (r_s = -0.406, P < 0.01).Conclusion1. Ezrin protein in poorly differentiated carcinoma tissue expression is significantly higher than that of well-differentiated carcinoma. And the expression of intensity and aggressiveness were positively correlated. Ezrin protein expression of Lymph node metastases is significantly higher than that without metastasis.2. FAK protein in lymph node metastases of colorectal cancer tissues is significantly higher than those without metastasis. Hints FAK protein may play a crucial role in tumor cell's invasion and metastasis. Abnormal expression of FAK protein is highly correlated with tumor's invasion and metastasis.3. With malignant degree increased, the expression of E-cadherin protein in colorectal cancer is significantly reduced. Suggest that E-cadherin protein may serve as a tumor invasion and tumor inhibitory factor plays an important role in the tumor's occurrence, development, invasion and transfer.4. Ezrin protein, FAK protein and E-cadherin protein may participate the biological functions such as the cell adhesion plaque formation, cytoskeleton assemble and link. Common-mediated and regulated the cells and cells with the extracellular matrix adhesion and in the process of tumor cell's invasion and metastasis. |
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