Expression And Clinical Significance Of FAK E-cadherin And LOX In Laryngeal Squamous Cell Carcinoma

Objective: Carcinoma of larynx is one of the most common malignant tumors in the head and neck tumors, it has a increasing tendency recently. Metastasis of carcinoma cell is a important factor of leading to patient's death. This complex process contain detachment from primarily focus, invasion into perienchyma, incycle of circulation system and lymphatic system, metabasis of distant organ and proliferation of new metabasis. The mechanism of invasion and metabasis of laryngeal carcinoma is not yet understood. We regard laryngeal squamous cell carcinoma as study object now, to explore the expression of FAK, E-cadherin and LOX gene in laryngeal squamous cell carcinoma and adjacent non-neoplastic tissues, to explore their role in the process of tumorigenesis, progression, invasion and metastasis of laryngeal squamous cell carcinoma and their correlation, to provide new theory and experiment evidence for pathogenesy, gene therapy and immunotherapy, clinical prognosis of the laryngeal squamous cell carcinoma.MethodsImmunohistochemistry was employed to measure the expression of FAK, E-cadherin and LOX in 51 specimens of laryngeal squamous cell carcinoma tissue and 22 specimens of adjacent non-neoplastic tissue. RT-PCR was employed to measure the expression of FAK, E-cadherin and LOX mRNA in 49 specimens of laryngeal squamous cell carcinoma tissue and 15 specimens of adjacent non-neoplastic tissue. SPSS 11.5 was applied to analyze the results of experiment.Results1 Expression of FAK and relation with clinical parameterImmunohistochemistry was employed to measure the expression of FAK in 51 specimens of laryngeal squamous cell carcinoma tissue and 22 specimens of adjacent non-neoplastic tissue. The positive expression rate of FAK protein in laryngeal squamous cell carcinoma tissues was 80.39% (41/51) , while that in the adjacent non-neoplastic tissues was 9.09%(2/22). There was significant difference in the positive rates of FAK protein between laryngeal squamous cell carcinoma tissues and the adjacent non-neoplastic tissues (P<0.01). RT-PCR was employed to measure the expression of FAK mRNA in 49 specimens of laryngeal squamous cell carcinoma tissue and 15 specimens of adjacent non-neoplastic tissue. In laryngeal squamous cell carcinoma tissues and the adjacent non-neoplastic tissues, the mean level of FAK mRNA were 0.66±0.29 and 0.32±0.20, the former was significantly higher than the latter (P<0.01). Results of IHC and RT-PCR showed that expression of FAK in laryngeal squamous cell carcinoma tissues was significantly higher than that in the adjacent non-neoplastic tissues (P<0.01). Expression of FAK wasn't correlated with age and pathological grading of tumor (P>0.05), but that was correlated with lymph node metastasis (P<0.05).2 Expression of E-cadherin and relation with clinical parameterImmunohistochemistry was employed to measure the expression of E-cadherin in 51 specimens of laryngeal squamous cell carcinoma tissue and 22 specimens of adjacent non-neoplastic tissue. The positive expression rate of E-cadherin protein in laryngeal squamous cell carcinoma tissues was 50.98% (26/51) , while that in the adjacent non-neoplastic tissues was 100.00%(22/22). There was significant difference in the positive rates of E-cadherin protein between laryngeal squamous cell carcinoma tissues and the adjacent non-neoplastic tissues (P<0.01). RT-PCR was employed to measure the expression of E-cadherin mRNA in 49 specimens of laryngeal squamous cell carcinoma tissue and 15 specimens of adjacent non-neoplastic tissue. In laryngeal squamous cell carcinoma tissues and the adjacent non-neoplastic tissues, the mean level of E-cadherin mRNA were 0.74±0.39 and 0.97±0.32, the former was significantly lower than the latter (P<0.05). Results of IHC and RT-PCR showed that expression of E-cadherin in laryngeal squamous cell carcinoma tissues was significantly lower than that in the adjacent non-neoplastic tissues (P<0.05). Expression of E-cadherin wasn't correlated with age (P>0.05), but that was correlated with pathological grading of the tumor (P<0.01) and lymph node metastasis (P<0.05).3 Expression of LOX and relation with clinical parameterImmunohistochemistry was employed to measure the expression of LOX in 51 specimens of laryngeal squamous cell carcinoma tissue and 22 specimens of adjacent non-neoplastic tissue. The positive expression rate of LOX protein in laryngeal squamous cell carcinoma tissues was 66.67% (34/51), while that in the adjacent non-neoplastic tissues was 100.00%(22/22). There was significant difference in the positive rates of LOX protein between laryngeal squamous cell carcinoma tissues and the adjacent non-neoplastic tissues (P<0.01). RT-PCR was employed to measure the expression of LOX mRNA in 49 specimens of laryngeal squamous cell carcinoma tissue and 15 specimens of adjacent non-neoplastic tissue. In laryngeal squamous cell carcinoma tissues and the adjacent non-neoplastic tissues, the mean level of LOX mRNA were 0.38±0.26 and 0.54±0.19, the former was significantly lower than the latter (P<0.05). Results of IHC and RT-PCR showed that expression of LOX in laryngeal squamous cell carcinoma tissues was significantly lower than that in the adjacent non-neoplastic tissues (P<0.01). Expression of LOX protein wasn't correlated with age (P>0.05), but that was correlated with pathological grading of tumor (P<0.01) and lymph node metastasis (P<0.01). Expression of LOX mRNA wasn't correlated with age and lymph node metastasis (P>0.05), but that was correlated with pathological grading of the tumor (P<0.05).4 Between mRNA level and protein level, There were negative correlations between FAK and E-cadherin expression in laryngeal squamous cell carcinoma tissues (r=-0.287, P<0.05; r=-0.287, P<0.05). There was a positive correlation between E-cadherin protein and LOX protein in laryngeal squamous cell carcinoma tissues (r=0.555, P<0.05). There were no correlations between FAK protein and LOX protein, between E-cadherin mRNA and LOX mRNA, between FAK mRNA and LOX mRNA in laryngeal squamous cell carcinoma tissues(r=-0.035, P>0.05; r=-0.023, P>0.05; r=-0.029, P>0.05).Conclusions1 The higher expression of FAK and the lower expression of E-cadherin and LOX were correlated with progression of laryngeal squamous cell carcinoma.2 The higher expression of FAK and the lower expression of E-cadherin and LOX were correlated with malignant biological behavior of laryngeal squamous cell carcinoma, which included lymph node metastasise and pathological grading of the tumor; these maybe collectively participated in a series of pathological progression (eg: progression, invasion and metastasis) of laryngeal squamous cell carcinoma.