Studies On Flavonoids From Leaves Of Murraya Exotical L

Murraya exotica L., a well-known medicinal plant,which is widely used for treatment of stomachic diseases, inflammation, different body pains, trauma , bleeding caused by internal and external injury etc. It was been also used as a tonic and haemostatic agent. Due to the demand for its important medicinal use in China, it was largely cultivated in Yunnan and Guangxi Province.The alkaloids and terpenoids in this plant have been reported more before, but the flavonoids reported fewer. For the sake of the better use of this herb medicine, this paper describes the isolation, structural determinations and the analysis of the flavonids from the dried leaves of Murraya exotica L.Extraction and SeparationDried leaves (1kg) were extracted with water (15L, 12L, 10L, respectively) for three times (2h, 1.5h, 1h, respectively). The extracted solution was absorbed by macroporous resin, then eluented with 95%EtOH. The extraction was subjected to Al₂O₃ column chromatography using ethylacetate as mobile phase and silica gel column chromatography using ethylacetate-petroleumether as mobile phase to afford .JA(11.2g),JB(8.6g),JC(1.3mg),JD(104mg),JE(11mg),JF(8mg)。Compounds JA, JB, JC, JD, and JE was identified respectively by UV, IR, MS, 1DNMR and 2DNMR.as. 5,7,3′,4′-tetramethoxyflavone;5,7,3′,4′,5′-pentamethoxyflavone ;5,6,7,3′,4′,5′-hexamethoxyflavone;3′-hydroxy-5,7,4′-trimethoxyflavone;7-hydroxy-5,3′,4′-trimethoxyflavone .Compound JF is not identified now and hoped to be identified in the future .But for JA and JC,all the compounds were isolated from Murraya exotica L. for the first time.Analysis of flavonoids in the leaves of Murraya exotica L.In this paper we present a more sensitive quantification method by HPLC for analyzing the main flavonoids (JA and JB) in the leaves of Murraya exotica L.HPLC was performed on a ZORBAX C18 column(250 mm×4.6 mm,5μm)at 25℃with the rate of 1.2 ml/min ,using 58% methanol and 42% wateras mobile phase.The content of the two flavonoids-compounds JA and JB in the Murraya exotica L. getting from different regions were determined by the above method, the results showed that their contents were variety in different regions. The content of JA and JB in leaves is higher than in the stems. The content of JA and JB extracted getting from Yunnan province was 1.58% and 1.29%.While those getting from Hebei province only was 0.54% and 1.68%.