Study On The Developmental Plasticity Of Neurons In The Visual Cortex Of Form Deprivation Amblyopia Rats

ObjectiveThe specific Nissle's staining techniques, immuno-histochemical and transmission electron microscopic methods were used to study the properties and correlated mechanisms of striate cortical cells in amblyopia rats, and to investigate the persistence of neuronal plasticity in the visual cortex of binocular deprivation amblyopia rats which has overgrown the critical period of visual development.Methods34 normal SD rats aged 13 postnatal days were randomly divided into three groups: normal ; non-illumination amblyopia; and illumination amblyopia group. Binocular tarsorrhaphy were enforced to the 27 amblyopia rats to set up the binocular deprivation amblyopia animal model. Rats of all three groups were reared together until postnatal day 100. After half an hour exposure to ambient light, the illumination amblyopia rats were perfused transaortically; and the other two groups were perfused directly. Sections of the OclM OclB areas of visual cortex were cut and then processed for c-fos immunohistochemistry and stained for Nissl substances. The impact of photostimulation (visual input) on the expression of c-fos protein in the visual cortex were compared; differentiation of morphological parameter of neurons in visual cortex together with results of c-fos immunohistochemistry between illumination amblyopia group and normal groups were analysed statistically. Ultramicrostructures such as nucleus, endochylema, rough endoplasmic reticulum,...