Studies On Chemical Constituents In The Seeds Of Panax Ginseng C.A.Mey. And Its Pharmacological Activities

In this paper, the chemical compositions and pharmacological activities of the seeds of Panax ginseng C.A.Mey. were studied.According to the references, many kinds of compounds has been isolated and identified, such as panaxsaponin,panaxan, essential oil,flavonoids,alkaloid,amino acids,vitamin,mineral element and so on. The modern pharmacological studies showed that Panax ginseng C.A.Mey. had the effects of anti-tiredness,improve remembrance,anti- athymia,mitigate effect, analgesic effect, anti- arhythmia, anti- tumor,anti-aging,anti-radiation etc.. In order to find out more chemical constituents which have bioactivities in the seed of Panax ginseng C.A.Mey. and develop more new drug products, our group gave a Systematic investigation on the seed of Panax ginseng C.A.Mey.. The research methods and results as follow: Firstly, pulverized and dried the seeds of Panax ginseng C.A.Mey. (0.5kg).Than, the powder were extracted with 70% ethanol and the extracts were concentrated in vacuo and got a ethanol parts(51.2g). Secondly, ethanol parts were divided into two parts.One was the ethanol part(25g),which which were dealt with silica gel chromatography by using several kinds of solvent systems and recrystallizing repeatly,got seven compounds.The other was extracted with n-butanol, concentrated in vacuo and got a n-butanol part. Than , this part was elute by 4020 resin for saponin. The saponin was also dealt with silica gel chromatography by using several kinds of solvent systems and recrystallizing repeatly , got three compounds.Among of them, the structures of seven compounds were established and identified on the basis of chemical and spectroscopic evidence(1HNMR , 13CNMR , 2DNMR). They are 20(S)-ginsenside Rg3(â… ), ginsenside Re(â…¡), ginsenside Rd(â…¢), 20( S)-ginsenside Rh2 (â…£), sucrose(â…¤), ginsenside Rc(â…¥),(z)-6-octadecenoic acid (â…¦).The antidiuresis of the ethanol extractive of the seeds of Panax ginseng C.A.Mey.,ginsenoside Rg3, ginsenoside Re , Panaxadiol Saponin,Panaxatriol Saponin. The results showed that the ethanol extractive of the seed of Panax ginseng C.A.Mey.,ginsenoside Rg3,Panaxadiol Saponin , Panaxatriol Saponin had conspicuous effect on antidiuresis.But the ginsenoside Re was weaker than other. At the same time, the effective of Panaxadiol Saponin and Panaxatriol Saponin are better than others. Than, with the kits method,the content of K+,Na+ and Cl- in urine collected were measured. The result indicated that the effective of antidiuresis relates to the drainage of K+.