A Study On The Effects Of The Components Of Nonessential Oils In Lavender Mint Leaves On Antioxidation And Protection Of Rat Glomerular Mesangial Cells Against Oxidative Damage

With the in-depth study of the foundational theory of the antioxidation and free radical and diseases, as revealed in defects of synthetic antioxidants intensely, make the natural efficient low toxic antioxidant substances began to be paid attention seriously which comes from nature, especially the Aromatic plants that have been Increasingly becoming the worldwide hot focus of research and the emphasis of venture investment due to their ability of outstanding antioxidation, For example mint of extensive application. Modern study have found, mint contains flavonoids, organic acids, amino acids and other active ingredients, which display remarkable activation of spasmolysis, acesodyne. anti-bacterial anti-virus and etc for the systems of life. However, international and domestic studies are focused on exploiture of essential oils, while we have limited systemic exploration of studies on the components of nonessential oils on which abilities for scavenging free radical, antioxidation and etc, especially for studies on the protection of oxidative damage of kidney tissue are rare.In the present study we choose the dry leaves of Lavender mint, after the essential oils of which have been extracted, it is the components of nonessential oils which are distilled by water, methanol-water ethyl acetate and ethanol that are taken as the experiment material, six chemiluminescence systems and two colorimetric systems are used to evaluate systemically their effect of scavenging directly free radical, inhibiting lipid peroxidation and preventing DNA oxidative damage caused by OH. Besides the cultured rat glomerular mesangial cells are taken as model, in which the effect of the activities of SOD and CAT and the concentration of GSH MDA and ONOO are evaluated by oxidative damage caused by H₂O₂ with water, methanol-distilled water and ethanol extractions, but also MTT and Flow cytometry methods are used to evaluate growth modality of GMC.The experiments of scavenging directly free radical indicate that the four different extractions of the components of nonessential oils in Lavender mint leaves can not only scavenge ^·O₂ ^·OH H₂O₂ ONOO DPPH^· and CO₃· but also has a better ability to inhibit lipid peroxidation and obviously mitigate or delay DNA oxidative damage caused by ^·OH.Results of cell model show that water, methanol-distilled water and ethanol extractions not only can promote prominently proliferation of GMC on low concentration while inhibit proliferation of GMC on middle/high concentration and influence cell cycling phase, but also can dose-dependently increase the activities of SOD CAT and the level of GSH , inhibit the production of ONOO and MDA in or in the cultured medium of the GMC which are damaged by H₂O₂. Above studies reveal that the components of nonessential oils in Lavender mint leaves can increase the oxidase activities and scavenge ROS and RNS directly, can prevent kidney tissue from oxidative damage, reduce production of LPO, maintain modality of glomerulus, inhibit cell proliferation, and possiblely alleviate or prevent GN ARF CRF DN and other kidney diseases.Above results indicate that the components of nonessential oils in Lavender mint leaves are very effective and multifunctional antioxidants and free radical scavengers with a good application prospect. These experiments also provide important applied gist for further exploitation of Lavender mint and other Aromatic plants, especially develop the function of them on medicine, nutrition and health care.