The Study Of The Effect Of Replenish Qi And Invigorate Spleen Decoction On Skeletal Muscle And Serum CK After Single Bout Of Exhaustive Swimming In Rats

PURPOSE This research according to TCM(Traditional Chinese Medicine) theory of zang-fu manifestation,Qi,Blood Body Fluid and TCM Injury of muscle and tendon theories, applying the instruction that the TCM Conception of holism and BZLZ(Bianzheng Lunzhi) thought cure adopt the traditional Chinese medicine prescription an appropriate improvement, observe Replenish Qi and Invigorate Spleen Decoction on the microstructural show of the skeleton muscle organization and serum CK variety After Single Bout of Exhaustive Swimming in Rats,inquirying in to Replenish Qi and Invigorate Spleen Decoction shows influence and its mechanism of the skeleton muscle microstructural show and the CK.METHODS object:Thirty rats,(Wistar,male,the weight is 200±20g,SPF) ;Replenish Qi and Invigorate Spleen Decoction.The mold method:Indoor(the environment temperature 23±s 4℃s) in the artificial weather, get the swimming method result in a model of Single Bout of Exhaustive Swimming in Rats, is assurance sport strength, shorten the swimming time, adopt usual burden heavy(about 7% of the weight, note:200 gs rat for 14gs)method to increase its sport quality and quantity. Divide Groups:Wistar rat is 30s, ising divided into 3 experimental groups by random:The A group is Sports Blank Matched Control Group, The B group is Sports Placebo Group, The C groups is Sports Chinese Medicine Decoction Group. Experimental methods:Thirty rats are go single bout of exhaustive swimming to build a mold after rats finish adaptability for a week swim to train after they were adaptability to feed 2 days.Give respectively, the A group put back normal feeding in cage after 2 hours, the B group and C group takes 0.9% NaCl and a Replenish Qi and Invigorate Spleen Decoction a group after 2 hours give respectively by infuse stomach,give to infuse stomach for the second time after 12 hours, after Single Bout of Exhaustive Swimming in Rats complete about 1 day,injected all rat with Anaesthetize a medicine(i.p).Then get the measureses of serum CK after get the blood, the instant takes random one the side gastrocnemius muscle, after fix, place to have a HE tissue slice(LIGHT MICROSCOPY).RESULT A,B and C group The CK analysis of variance result can see, being to show the result is (P<0.05) ,asα=0.05, Three groups is statisticsed to learn markedly.When the level asα=0.05, and carry ont the A group and C group take a t-test, manifestation(P<0.05), that difference has already statisticsed to learn meaning.The B group and C group take a t-test, manifestation(P<0.05), that difference has already statisticsed to learn meaning.But when the level asα=0.05, the A group and the B group examine by t-test, manifestation(P>0.05) that difference didn't statistics to learn meaning. the rat gastrocnemius muscle adoption of is the HE tissue slice(LIGHT MICROSCOPY), result three groups specimen contrasts didn't see obvious difference.The specimen abnormality is limited by the tissue edema of the skeleton muscle only, and the tissue edema circumstance compare slightly, other structure saw unusually.