MRNA Differential Display In Peripheral Blood Leucocyte Of Uigur And Kazak Patients With Type 2 Diabetes Mellitus

Objective: To search the differentially expressed genes in Uigur and Kazak patients with and without type 2 diabetes mellitus. Methods: The differentially expressed cDNA bands were isolated by fluorescent mRNA differential display from peripheral blood leucocyte of the Uigur and Kazak patients with type 2 diabetes mellitus and the normal controls. After being cloned, all cDNA fragments were sequenced, then underwent sequence analysis, homogenous comparison, and dot hybridization. Result: Z5,Z8,Z15 differentially expressed cDNA fragments were found.They were over-expressed in the normal controls and were lower or scarced in the Uigur and Kazak patients with type 2 diabetes mellitus. They were selected for sequencing and hybridization. They showed highly homologous to some regions of Human chromosome 1 and 14, but the fragments functions are unknown. Conclusion: The three differentially expressed genes might have a relation with type 2 diabetes mellitus.The developed fluorescent mRNA differential display technique was a simple, smart and efficient method for screening differential expressed genes.