The Quantitative Analysis And Application Of Cell-free DNA In Maternal Plasma

About 4 percent of newborn bodies are affected by one or more kinds of congenital diseases. And, there are about 35 percent of congenital diseases belong to inherited diseases. Forever, it is significant to prevent and control efficiently to the incident and mortality of new body that acquiring the fetal information for prenatal diagnosis in time. Nowadays, genetic analysis of fetal information is routinely performed from chorinonic villus samples, umbilical cord blood or by amniocentesis, these approaches involve invasive procedures with increased risk of both mother and fetal, it cannot be accepted by the mother. To avoid such complications, attempts have been made to develop non-invasive tests. Relatively, the discovery and isolation of the presence of large amounts of cell-free fetal DNA in maternal blood represents a promising noninvasive approach to prenatal diagnosis.In order to establish the methods of fluorescent quantitative PCR for the measure of the GAPDH(a hose keeper gene) and the sex determining region Y(S R Y) gene; to investigate the fragment-length distribution and the change of plasma DNA among the different trimester of pregnancy and between the normal and abnormal pregnancy; to measure the quantity and the change of the plasma DNA of different trimester of pregnancy,...