Effectiveness Of Liposomal Honokiol Combined With Hyperthermia In Mice Lewis Lung Cancer In Vivo

Honokiol (HNK) is an active component purified from Magnolia, a plant used in traditional Chinese herbs . It is known to possess potential anti-tumor effect mainly by inducing apoptosis and inhibiting angiogenesis. Hyperthermia(HT), an important treatment regimn following traditional treatment regimns such as surgical therpy, radiotherapy, chemotherpy and biological therpy, kills tumors by changing the fluidity and permeability of cell membrane,denaturating intracellular proteins, inducing apoptosis and inhibiting angiogenesis. The present study was designed to determine whether hyperthermia enhances the antitumor activity of loposomal honokiol in vivo.Lewis lung carcinoma (LLC) cells treated with Lipo-HNK plus HT, displayed the apparently increased apoptotic cells and antiangiogenesis compared with treatment with Lipo-HNK or HT alone. Mice bearing LLC were treated with intraperitoneal injections of Lipo-HNK (0.5mg every day for 20 days) plus HT (42℃,one hour every five days). Combined treatment with Lipo-HNK and HT significantly reduced mean tumor volumes compared with either treatment alone in LLC lung cancer model and prolonged survival time.