Mechanisms Of The Expression Of Exogenous Genes Injected Via Duct In Mammary Gland Tissue

Animal mammary gland bioreactor is part of transgenic animal, which has been made great progress during the past decades since the transgenic technology was created. It has good application prospects. The production of different pharmaceutically important human proteins in the mammary gland of transgenic animals constitutes an important field of modern biotechnology. The technology of injection via duct into mammary gland can be used to make the mammary gland bioreactor. To study mechanisms of the expression of exogenous genes injected via rat's duct in mammary gland, and to explore a way to increase the transgenic efficiency, this experiment is designed.There are three parts in the experiment. In the first part, paraffin sectioning of the female rats' mammary gland and H-E staining were made during 8 stages of mammary growth (including 4 weeks old, 9 weeks old, pregnancy 7 days, pregnancy 12 days, pregnancy 18 days, post partum 7 days, post partum 14 days, post partum 21 days). The configuration changes of the mammary gland tissue were observed by light microscope. The rat's mammary gland was compound tubulo-acinous gland. The morphology changes of the rat mammary gland tissue were most evident from pregnancy 12 days to post partum 14 days. During this stage a lot of serous gland cells were proliferating. Compared to other days, on pregnancy 12th day and post partum 7th day more gland cells were in division. The rat's mammary growth was...