Apply The Single Tube PCR Amplification Assays To Analyse The HLA-DRB1 Gene Polymorphism Of Chengdu Hans.

[BACKGROUND & AIM] The human major histocompatibility complex (MHC) , also called the human leucocyte antigen( HLA), is currently known as the most complicated heredity polymorphism system, and it is the main gene system adjusting the human body special immunity and determining the individual disease susceptibility . It is widely used in the fields of anthropology, forensic, immunology, organ transplantation and disease relativity etc.The HLA has the most polymorphism, and its gene distributing is obviously different in different ethnic groups, nationalities and geographical areas , which results in the distinct difference of frequency of some genes among the different nationalities. So before studying HLA, we must investigate the groups and establish the gene bank in different nationalities .Up to now the research of Chinese different groups on HLA local polymorphism and distributing frequency is not sufficient, and also Sichuan Han' HLA-DRB1 gene typing data is absolutely blank. About the HLA research, there are various gene typing methods based on PCR, which are progressive comparing to the methods of serology and cytology . But they are still complicated, so it is necessary to look for...