Fulminant Acute Pancreatitis Induced By L-arginine In Rats

Background and object:Fulminant acute pancreatitis(FAP) became as a especial acute pancreatitis(AP), it is in fact a severe acute pancreatitis(SAP) with acute depravation in the early state. In clinic, it was a sudden and extremely dangerous disease. Most organ disfunction failure appeared in the early stage of FAP, and the mortality rate was very high. Though there were reports with the pathogeny and treatment in FAP, there was limited studying in FAP and other organs in the world. Therefore, it was significant to establish a animal model with little trauctic to systemly explore the FAP and the injury of other organs, and then decreased the mortality. This experiment was aimed to explore the establish of FAP in rats with L-arginine and new fashion of injection.Methods:144 Wistar rats were randomly divided into experimental group(L-Arg group, n=72) and control group(NS group,n=72). L-Arg group received intraperitoneal injection of 450mg/100g body mass and subcutaneous injection of 150mg/100g body mass with L-arginine at an interval of 2h. NS group received the same volume of 0.9% sodium chloride in the same way. After the last injection, 30 rats were obtained individually in L-Arg and NS group, and the mortality of rats within 0h-120h in both group was measured. At the every point of 0,6,12,24,48,72 and 120h, six rats were obtained in L-Arg and NS group, and the following index were tested in both group: the serum amylase and serum lipse; the ratio of wet/dry with pancreas; the function of liver, kidney ang lung, and the routine of blood. The pathological changes of pancreas, liver, kidney and lung were observed with HE dying at the same points, and then gave them pathological scores or grade.Results:After the intraperitoneal and subcutaneous injection at an interval of 2h in both group, the following Results were received.(1) The rats in L-Arg group were died 0 at 0-12h, and one at 6-12h, 2 at 12-24h, 7 at 24-48h, 5 at 48-72h, one at 72-120h, the mortality within 120h was 53.33%. There was no rat died in NS group within 120h.(2) The serum amylase in L-Arg group was increased at 6h, and peaked at...