Protective Effects Of Edaravone On Oxidative Injure Of Endothelial Cells Induced By Hydrogen Peroxide And Its' Possible Mechanism

Objective To investigate the protective effect of Edaravone( MCI-186) on oxidative injure of endothelial cells induced by H₂O₂;to examine the effect of MCI-186 on the expression of ICAM-1mRNA and MCP-1 mRNA and to study the effects of MCI-186 in atherosclerosis,the possible mechanism of its antiatherosclerosis.Methods In order to gain the oxidative injure cell model,we treated endothelial cells with H₂O₂and observed the oxidative injure of cells after treated by the different concentrations H₂O₂ as well as after treated by the same concentration H₂O₂ for different times;Endothelial cells were incubated with MCI-186 and H₂O₂ and then the protective effect of different concentrations MCI-186 on the oxidative injure of endothelial cells was elucidated,the expression ofICAM-1 mRNA and MCP-1 mRNA was observed;the cell growth condition was investigated with inverted microscope; the viability of cells was determined by MTT assay;the level of cells lipid peroxidation was detected by measuring MDA ;the expression of ICAM-1mRNA and MCP-1 mRNA was measured by RT-PCR.Results MCI-186 can decrease the extent of contracted endothelial cells damaged by H₂O₂; MCI-186 can diminish the level of MDA that was produced because of the oxidative injure of endothelial cells; the viability of EC can be improved by MCI-186; RT-PCR showed that ICAM-1 and MCP-1mRNA was expressed in all groups.Higher level of ICAM-1 and MCP-1mRNA expression induced by H₂O₂ was observed in H₂O₂ Stimulated group compared with that in control group (P<0.05).After being treated with MCI-186,The expression of ICAM-1 and MCP-1mRNA in H₂O₂+ MCI-186 group was reduced (P<0.05 ).Conclusions (1) The ICAM-1 and MCP-1mRNA incultured endothelial cells is markedly induced by H₂O₂(2)MCI-186 could decrease the oxidative injure of endothelial cells induced by H₂O₂;(3) MCI-186 could inhibit the expressions of ICAM-1 and MCP-1 mRNA induced by H₂O₂ .That may be one of the mechanisms of antiatherosclerotic effect of MCI-186.