Dan Joan Jade Particle Impact On The Model Mouse Salivary Gland In Sjogren's Syndrome

Background: sjogren syndrome(SS) is a familiar disease which impinges exocrine glands.It is mostly relate to immunity maladjustment.Most clinic doctors treat SS by means of modulation immunity maladjustment and facilitate excrete of exocrine glands,but this kind of medicaments have a incertitude curative effect and side-effect of vary extents,and most old folks are prone to suffer from SS.Those patients are tend to intercurrent of maligancy.It is sense of looking for those druggery which have unconspicuous side-effect and affirmative curative effect.There has a understand to SS of long standing in the domain of tradition Chinese medicine.Herbalist doctors have abundance of experience and relatively integrate theoretics in the facet of treating SS.Chinese traditional medicines are affluent and have unconspicuous side-effect.Many Chinese traditional medicines are capable of modulation immunity maladjustment which is bidirectional.It is think much of treating SS by way of Chinese traditional medicines. Dan Di Qiong Yu Granule is a experience patent medicine which is used in treating SS by my tutor.The past research make clear:Radix Ginsen, Rsdix Salvia Miltiorrhizae, Radix Rehmanniae, Radix Ophiopogonis are capable of modulation immunity maladjustment which is bidirectional, and of resist to inflammation. These Chinese traditional medicines are central component of Dan Di Qiong Yu Granule.Objective: To exploituring new patterm of medicine which is capable of treating SS,we probed into Dan Di Qiong Yu Granule's impact to salivary gland of SS mice and part of therapeutic mechanism,by way of research achievement in the field of western medicine and advanced experimentation technique. To investigate the terapeutic efficacy andmechanism of Dan Di Qiong Yu Granule on sjogren syndrome(SS) mice.Methods: Fist,coarse antigen was cranked out by way of grinding ICR mice's mandible glands;Second,mix liquid was corned into being by way of mixing thyroxin and reserpine;Last,we injeted the coarse antigen into ICR mice's hypodermal and fill the mix liquid into ICR mice's stomach.So sjogren syndrome mice model was come forth,and those mice were random diveded into A B C D E groups.Accordingly,NS, Liu Wei Di Huang Granule, three different dose (low,middle and high) Dan Di Qiong Yu Granule were administered to SS mice.After 28 days, using NS as negative contrast, Liu Wei Di Huang Granule as positive contrast ,we studied the effect of Dan Di Qiong Yu Granule on SS mice,such as their salivary flow by weigh in gauze which park under mice'stongue, CD4~+T cells, CD4~+/CD8~+ by way of FCM, their autologous antibodies by way of bidirectional immunity diffuse,there mandible glands organise slice and there daily average watering capacity in last weeks.Results:1.In the test of doing sjogren syndrome mice model,we found that each mice's salivary flow in group of doing SS mice was fewer than each mice's salivary flow in group of NS,their difference was distinct(p<0.05). Each SS mice was more than NS mice in daily average watering capacity in last weeks,it indicates this method can boost up mice's sense of thirstily.By measure of precise probability,there has distinct difference in the autologous antibodies positive rate between SS mice and NS mice ,so does in the positive rate of lymphocyte sokage into mandible glands organise slice ,and so does CD4~+T cells, CD4~+/CD8~+(p <0.01).2. In the test of the effect of Dan Di Qiong Yu Granule on sjogrensyndrome mice's salivary gland,we found that it has statistic sense in daily average watering capacity between in Dan Di Qiong Yu Granule groups(low,middle and high) , Liu Wei Di Huang Granule and NS group after therapy(p<0.05),so does Dan Di Qiong Yu Granule groups(low,middle and high) and Liu Wei Di Huang Granule groups. There has distinct difference in CD4~+T cells, CD4~+/CD8~+ between Dan Di Qiong Yu Granule groups(low,middle and high) and Liu Wei Di Huang Granule group(p <0.01).In mice s salivary flow, Dan Di Qiong Yu Granule groups(low,middle and high) have statistic sense with other groups(p <0.05...