Expression Of C-met Stimulated By High Glucose In Human Renal Tubular Epithelial Cell And Its Significance

Objective To abserve high glucose-induced expression of c-met mRNA in human renal tubular epithelial cell (HK-2),and to investigate the role of HGF/c-met system in diabetic nephropathy.Methods A cell culture system of human kidney-2(HK-2) was used to study. The cellure proliferation in media containing high concentration of glucose(25mmol/L) or HGF(50ng/ml) was assessed using MTT. Levers of HGF and c-met mRNA were examined at multiple time points with RT-PCR assay.Results After 24h culture, when compared to the control group, high glucose inhibited cellular proliferation ,exogenous HGF induced cellular proliferation(P<0.05).Expression of c-met mRNA was detectable at the 12th hour and mounted to the peak at the 24th hour after HK-2 incubated in high-glucose culture(vs normal glucose control, P<0.05).Co-incubation of exogenous HGF with HK-2 in high-glucose culture for 48 hours increased c-met mRNA expression significantly as compared to the high glucose control(P<0.01). Producation of endogenous HGF mRNA was detectable at the 6th hour and mounted to the peak at the 12th hour after incubated in high-glucose culture(vs normal glucose control, P<0.05).Conclusion In vitro, high glucose inhibited HK-2 cells proliferation, and induced cells apoptosis in long time cultured, and with the continuous stimulation of high glucose,the expression of HGF,c-met mRNA in HK-2 cells was decreased gradually. Exogenous HGF promotes cellular proliferation in media containing high glucose, and induces its receptor c-met higher expression. The results are probably associated with that local up-regulation of c-met is crucial to renal repair in diabetic nephropathy because of increasing activity of HGF/c-met system.