| Objective To investigate the immune tolerance induced by allogeneic spleocytes in cardiac allograft of rats, and study mechanism concerning transplantation tolerance, and compare the effects of immunological tolerance induced by administration of allograft splenocytes in different times.Methods Heart transplantation operation were carried out between SD and Wistar rats, and allogeneic spleen cells in an amount of 2×10~8 were injected via portal vein during the operation. Cyclophosphamide was injected intraperitoneally in a dose of 80mg/kg two days later; or allogeneic spleen cells in an amount of 2×10~8 were injected into portal vein, and then heart transplantations were carried out one week later. The survival times of allograft were observed and monitore,and the immunological states of recipient were evaluated. Allograft in one rat of each groups was examined under light microscopy in the six day after heart transplantation. To observe the immune tolerance status resisting another allograft,the rats in the tolerance-inducted groups were transplanted with skin from SD rats in thirty days later.Result The mean survival times (MST) of cardiac allograft were 30.17±2.79d in the group injected splenocytes during the operation, and were 30.67±3.44d in the group injected splenocytes before operation. MST were longer than that of the control in both of the groups(P<0.01). Compared with the control group, the alterations on histopathology in cardiac allograft were slightly mild in the tolerance-induced groups.The measurement for CD25+ T lymphocytes showed it was in the lower transplantation reaction level after administration of allogeneic splenocytes. However, the tolerance couldn't be induced, to the...
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