Study On Association Of The Beta3-adrenergic Receptor Gene With Karzak Obese Children In Xinjiang

Objective : To investigate the association between Trp64Arg mutation of theβ3-adrenergic-receptor gene and obesity in Karzak children. Methods: 95 patients with obesity and 87 controls were selected from the area around Urumqi.Genotype of the β3-AR was determined by polymerase chain reaction(PCR)and restriction fragment length polymorphism methods (RFLP).Polymorphism(PCR RFLP)and serum concentration of triglyceride,total cholesterol,low density lipoprotein cholesterol (LDL-C),apolipoprotein A(ApoAI),apolipoprotein B(ApoB) and high density lipoprotein cholesterol (HDL-C) were measured with biochemical method.In the mean time ,the weight and height were detected. Results: The mutative Callee was 0.194 for all the children,0.20 for boys and 0.16 for girls. The frequency of callee and the genotype Trp64Arg was higher in obese children . There were higher levels of the lipids in Karzak obese children than in the control group,excluding apolipoprotein A and high density lipoprotein cholesterol. There was no apparent difference between the genotype Trp64Arg and Trp64Trp in serum concentration of lipids and body mass index(BMI). Conclusions: There was distribution of β3-AR Trp64Arg mutation in Karzak school-aged children in XinJiang.These results suggest that the Trp64Arg mutation of thep3-adrenergic-receptor gene may be associated with Karzak children obesity.