Study Of Relevant Risk Factor And Molecular Epidemiology On Polymorphism Of UCP2 Gene In Children Simple Obesity

Obesity is a kind of nutrition metabolized disease. As a result of thechanges of the lifestyle, the incidence of obesity among children has beenincreasing year after year. In the primary 50 years of 21 century,preventing the prevalence of obesity will become one of the biggestchallenges in the public health. Children simple obesity has beenconsidered to be one of the most important issues of children's health inthe world.Obesity is the combined effects of heredity and environment. Genesdecided the susceptibility of the weight gaining. While the environmental factors including food intake and activity codetermined the balance ofenergy. A population-based molecular epidemiological study of childrensimple obesity was conducted in NanTong of Jiangsu Province, whichinvestigated the effects of heredity and environment on children simpleobesity, and provided the strategies and measures to prevent childrensimple obesity.PARTⅠThe Effects of variation of uncoupling protein-2and theβ3-adrenergic-receptor genes on children simple obesityObjective To investigate the correlation of children simple obesity withuncoupling protein 2 (UCP2) gene Ala55Val variation andβ3-adrenergicreceptor(β3-AR) gene Trp64Arg variation, and the combined effects ofthe two genes variation on children simple obesity. MethodsPCR-RFLP was used to detect the UCP2 gene Ala55Val variation andβ3-AR gene Trp64Arg variation. The allele frequency distributionsbetween case group and control group, and the differences of BMI andbiochemical indexes between genotypes, were analyzed. Results(1)Without consideringβ3-AR gene Trp64Arg variation, The frequencyof UCP2 gene Ala55Val variation in children simple obese subjects wassignificantly different from that in control subjects(P=0.001). (2) Withoutconsidering UCP2 gene Ala55Val variation, The frequency ofβ3-AR gene Trp64Arg variation in children simple obese subjects wassignificantly different from that in control subjects (P=0.014). (3) Whenthere was onlyβ3-AR gene mutation, the frequency of gene mutation inchildren simple obese subjects was not significantly different from that incontrol subjects; While there was only UCP2 gene mutation, thefrequency of gene mutation in children simple obese subjects wassignificantly different from that in control subj ects(OR=2.116,95％CI1.248-3.588). But when there were simultaneously two genes mutations,the frequency of gene mutations was higher in children simple obesesubjects than in control subjects (OR=4.069, 95％CI 2.057-8.050).Conclusion The accumulated effects of the two micro-genes increasedthe risk of the occurrence of children simple obesity.PARTⅡMolecular Epidemiological Study on the Risk of children simpleobesityObjective To study the prevalence and risk factors of simple obesityamong children in Nantong, and to provide evidence for the interventionmeasures against children's obesity. Methods The simple obesecases from a cluster random sample of 2461 children aged 2-12 were tested by a physical examination. A case-control study was conducted toanalyze the risk factors of simple obesity. The risk factors were collectedthrough the questionnaire, including family background, history offamilial obesity, situation of birth and feeding patterns, history of disease,living style and psychology of children. Results Among 2641children, 173 were diagnosed as simple obesity, and the prevalence ratewas 6.55％, 7.24％(100 children) for boys and 5.80％(73 children) forgirls. The prevalence rates of slight, moderate and serious obesity inchildren aged 2-6 was 3.51％, 1.25％and 0.79％respectively. Theprevalence rate of simple obese in children aged 7-12 was 7.05％. Therate of children simple obesity was affected by such factors as history offamilial obesity(P=0.012), parents eating snacks(P=0.050), parturitionmode (P=0.011),feeding style(P=0.038), duration of breastfeeding(P=0.011), parents'opinions of food intake(P=0.000), passiveeating(P=0.000), taste of eating(P=0.067), selective eating(P=0.008),eating speed(P=0.000), eating quality(P=0.050), appetite atpresent(P=0.000), appetite in the past(P=0.000), excessive intake offood(P=0.000), eating with playing frequently(P=0.013), eating with nohunger(P=0.002), eating fatness food(P=0.005), the time of outdoorsactivity(P=0.014), restlessness(P=0.029), the children's attitude tofigure(P=0.000); Our study also suggested, the risk of children simpleobesity was increased by the interaction between, UCP2 gene variation or β3-adrenergic receptor(β3-AR) gene variation, and environment. Theresults of non-conditional logistic regression analysis indicated thathistory of familial obesity, parturition mode, duration of breast feeding,eating speed, appetite at present, appetite in the past, excessive intake offood, eating fatness food and the children's attitude to figure werecorrelated to obesity. Conclusion Health education on sound lifestyle and eating habits should be emphasized to induce the incidence ofobesity in children.