Experimental Study On The Effects Of Dicha Kechuan Oral Liquid On The Antioxidative Enzymes' Activity And Maleic Dialdehyde Contents In Serum And Lung Tissues Of Mice With Smoking Induced Chronic Bronchitis

ObjectiveChronic bronchitis is one kind non-distinctive inflammation of tracheae, bronchial mucosa and surrounding tissues. One research considers that the imbalance of oxidant-antioxidant system makes an important promotive role during the forming and developing of chronic bronchitis. Another research indicates that Chinese medicine preparation-Dicha Kechuan Oral Liquid (DKOL) has a good curative effect on chronic bronchitis during both the acute outbreak stage and chronic deferment stage, for its function of resolving phlegm and relieving coughing, diminishing inflammation and appeasing asthma. On the basis of these points, and in order to provide the science experimental basis for this preparation's further clinical practice, development and promotion, the experiment uses the model mice with smoking induced chronic bronchitis to research the effect of DKOL on the activity of antioxidative enzymes-superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and the contents of maleic dialdehyde (MDA) in serum and lung tissues, and then study the possible mechanism of treating chronic bronchitis with DKOL from the antioxidant perspective. MethodsThe 60 mice are divided into 6 groups at random: untreated normal contrastive group, model contrastive group, DKOL high dose group, DKOL middle dose group, DKOL low dose group and CMTTFB group. The chronic bronchitis animal models are duplicated by refined smoking-fumigated method. The mice of the untreated normal contrastive group are conventional raised, and the mice of the model contrastive group aren't treated with any medicine, the other 4 smoking groups are intervened respectively with high dose, middle dose, low dose of DKOL as well as CMTTFB, which treat from the 1st smoking day. After completing the model duplication, we determine the SOD & GSH-Px activity and MDA contents in serum and lung t issues of each group, and at the same t ime, stochastically select two mice's partial lung tissues in each group, and make the pathological sections with HE staining, then use the light microscope to observe the pathology configuration changes of the lung tissues and take photographs. At last, we deal with the obtained data and analysis the pathological pictures to draw conclusions.