Studies On Antidiabetic Effects And The Mechanism Of MT

In this paper, the antidiabetic effects and the mechanism of MT in the levels of animals and cells were investigated. We established diabetic model rats induced by alloxan, streptotocin (STZ) and adnephrin, in order to investigate the effect of the decreasing glucose, triglyceride(TG) and increasing the content of insulin. The results suggest that MT can significantly decrease the level of glucose and TG while increase the content of insulin. In the experiments of normal mice, MT can significantly decrease the level of glucose, while increase the level of hepatic glycogen.The studies of islet cell line NIT-1 cells showed that SNP, alloxan and amylin were able to significantly decrease the cell activity ratio and SOD level, while increase NO and MDA production, reduce insulin release at glucose stimulation. When incubated with different concentrations of MT, the injury experiments of SNP, alloxan and amylin-induced NIT-1 cells were reversed by remarkably inhibit the decrease of cell viability, reduce NO and MDA production and enhance SOD level and increase the level of insulin. So it suggests that MT may play a protective role in NIT-1 cells injury induced by SNP, alloxan and amylin. The experimental studies of HepG2 cell showed that MT can significantly improve the IR situation induced by high content insulin.In conclusion, the present results indicated that MT showed antidiabetic effects and could improve the damage of NIT-1 cells induced by variable stimulants. The mechanisms may be related to the protective effects of MT on the injuried NIT-1 cells induced by SNP, alloxan and amylin. In addition, the effects of MT may also related to improve the condition of insulin resistance(IR).