The Protective Effect And Target Extrect Of YJH On Diabetes

To investigate the antidiabetic effects and the mechnism of YJH in the levels of animals and cells . we established diabetic model rats induced by streptotocin (STZ) and alloxan , while use the spontaneous diabetic KK mice , in order to investigate effect of the decreasing glucose and GSP and increasing the concentration of insulin .The results sugest YJH can significantly decrease the level of glucose and GSP while inrease the concentration of insulin in the experiments of STZ-induced rats model , In the experiments of AXN-induced rats model , YJH can significantly decrease the level of glucose, while YJH can decrease the level of glucose in the spontaneous KK mice . The studies of islet cells experiments showed that SPN , alloxan and amylin was able to significantly decrease SOD level,increase nitric oxide(NO) and MDA production , reduce insulin release at glucose stimulation , decrease DNA concentration in islet cells . When incubated with different concentrations YJH , the injury experiments of SPN-induced , alloxan-induced and amylin-induced B-cells was reversed by reducing NO and MDA production and enhancing SOD level. So it suggest that YJH may play a protective role in B-cell injury induced by SPN , alloxan and amylin-induced a significant decrease in SOD activity , inhabit insulin and DNA concentration synthesized , while increase MDA profuction and NO production . When incubated with different concentrations YJH , the injury of SPN , alloxan amylin-induced B-cells was reversed by reducing NO and MDA production and enhancing SOD level., insulin and DNA concentration . Pancreatic islet cells from SD rats were cultured in vitro. Pancreatic islet cells were incubated with exogenous nitric oxide , alloxan and amylin and different concentrations YJH . The experimental studies of HepG2 cell explored that YJH can significantly improve the IR situation induced by high content insulin .From the results of the animals , it is indicated that YJH possesses obvious protective effect against AXN and STZ induced artificial diabetic animal , at the same time YJH...