Effects Of Pentabrominated Diphenyl Ethers On The Structure And Function Of Thyroid And Mechanism In Rats

Objective: To study the effects of pentabrominated diphenyl ethers on the structure and function of the thyroid in rats and mechanism.Methods: In time-effect study, 100 health 4 weeks old SD rats were divided into 10 groups randomly, each sex of rats was 5 in each group, infected 10,15,20,30 and 40 days in the experimental and control groups respectively; Treated rats were gavaged daily with 60mg/kg BDE-99 in corn oil according to 0.5% body weight. Serum free T₄ , T₃ and TSH were measured in duplicate by standard radioimmunoassay kits, the pathology changes of thyroid tissue were observed by hematoxylin-eosin(HE) dyeing, and PCNA expression was examined by immunity histochemistry. In dose-effect study, 40 health 4 weeks old SD white rats were divided into 4 groups randomly, each sex of rats was 5 in each group; The treated rats were orally administered various doses of BDE-99(30,60,120mg/kg),we examined the change of serum hormone , histopathology , histochemistry and follicular cells ultramicroscopic structure after 15 days.Results: Time-effect study showed: Serum FT₄,FT₃ and TSH concentration...