Expression Of Skp2,p27 And Caspase-3 Protein In Endometrial Carcinoma And Their Affects On The Proliferation And Apoptosis Of Carcinoma Cells

Objective To detect the expression levels of Skp2,p27,caspase-3 and Ki-67 in endometrial carcinoma,atypical hyperplasia endometrium and normal endometrial tissues. Their association with clinicopathological characteristics of endometrial carcinoma was then analyzed. We explored the relationship of Skp2 and p27 expression and their effects on proliferation and apoptosis of endometrial carcinoma cells. To discuss their roles in the genisis and development of endometrial carcinoma .Methods Immunohistochemical Sp technique was used to detect the expressions of Skp2,p27,caspase-3 and Ki-67 in 62 cases of endometrial carcinomas,15 cases of atipycal hyperplasia and 32 cases of normal endometrial tissues. We detected the apoptosis of carcinoma cells with in situ end labeling technique to observe the morphology of apoptosis cells with light microscope.Results The positive rate of Skp2 expression in endometrial carcinoma was 79.0%,remarkably higher than 60.0% in atipycal hyperplasia and 31.2% in normal endometrium with a difference of statistical significance(p<0.05). The positive rate of p27 expression in normal endometrium,atipycal hyperplasia and endometrial carcinoma were 88.4%,60.0% and 58.1%. There was a tendency of low expression of p27 in endometrial carcinoma. Both the positive rate of Skp2 and p27 expression were correlated with the differentiation,invasion depth and lymphonode metastasis(p<0.05). But there were no significant difference correlated with clinical stage and pathological types(p>0.05).p27 expression was negatively correlated with the expression of Skp2 in endometrial carcinoma with significant difference statistically( r= -0.291,p= 0.022). But no significant correlationship of Skp2 and...