The Effect Of Protein Kinase 2 Inhibitor CX4945 On Proliferation, Apoptosis And Angiogenesis In Endometrial Cancer

Objective:To explore the effect of protein kinase CK2 inhibitor CX4945 of proliferation and apoptosis of endometrial carcinoma cell line IshikawaMethod:1. CCK8 method was used to detect different concentrations of CX4945(0,2,4,8,16,32,64,100)umol/L the role of 24 hours a day for Ishikawa endometrial cancer cells inhibition rate, using graphpad prism5 software to calculate the inhibition rate for 50% of the CX4945 concentration that IC50.2. The use of different concentrations of CX4945(0,5,10,20,30)umol/L in endometrial carcinoma Ishikawa cells 4 hours, 24 hours, 48 hours after Western blot to detect p-AKT and VEGFA and Caspase3 protein expression changes.3. Proliferation ability was detected on Ishkawa cells by scratch assay after CX4945(30umol/L) treatment4. Apoptosis was Hoechst33258 staining method to observe the changes of CX4945(0,30umol/L) nucleus 24 hours after Ishikawa cells, detection.Result:1. CX4945 for inhibiting human endometrial cancer cell line Ishikawa IC50 was 54.13umol/L.2. Different concentrations of CX4945(0,5,10,20,30)umol/L role to endometrial Ishikawa cells 4h, 12 h after p-AKT Th308 ser473 in the protein expression with the increase of drug concentration and reduced protein expression decreased with the prolonging of time and reduced(P < 0.05).3. Different concentrations of CX4945(0,5,10,20,30umol/L) in Ishikawa cells of 48 h in endometrial carcinoma, the expression of VEGFA protein decreased with the increase of the concentration of(p<0.05).4. Different concentrations of CX4945(0,5,10,30umol/L) on apoptosis protein expression of Caspase3 increased with the increase of concentration of Ishikawa cells 24 h after endometrial cancer(p<0.05). With the expression of apoptosis protein Caspase3 protein increased, cell apoptosis significantly increased compared with the control group(p<0.05).5.The migration of endometrial cancer cells Ishikawa, CX4945 effect was significantly less than the control group.Conclusion:1.Proliferation and apoptosis of endometrial cancer were studied by CX4945.2.Up-regulated expression of Caspase3 and down-regulated expression of p-Akt suggested that inhibition of proliferation and promotion of apoptosis may be through PI3K/AKT pathway by protein kinase CK2 inhibition.3. Expression of VEGFA protein restrained by protein kinase CK2 inhibition hinted that it may supress angiogenesis of endometrial cancer.