An Experimental Study On The Effect Of Cryopreservation Acting On Vascularized Bone Allotransplantation

Objective To investigate the effect of cryopreservation on the antigenicity of vascularized bone allotransplantation ,and observe the patency of the pedicle and the osteosis of bone allografts, and find out the dose of immunosuppressive agent.Methods 60 New Zealand rabbits were involved in the study, among these 20 rabbits were served as donors, 40 rabbits were hosts. Allotransplantation of cryopreserved radius diaphysis with the vascular pedicles were implanted to 8 rabbits and immunosuppressive agent(CsA 10mg/kg/d) was administrated 4 weeks after transplantation (group A ), similar cryopreserved grafts with the vascular pedicles were implanted to 8 rabbits and immunosuppressive agent(CsA 5mg/kg/d) was administrated 4 weeks after transplantation (group B ) , similar cryopreserved grafts with the vascular pedicles were implanted to 8 rabbits and immunosuppressive agent(CsA 2mg/kg/d) was administrated 4 weeks after transplantation (group C ) , allotransplantation of cryopreserved radius diaphysis with a vascular pedicle was performed in 8 rabbits(group D) ,8 rabbits received allotransplantation of fresh vascularized long bone were also compared(group E), 20 donors were group F. Immunological analysis of was performed at 1,4 week. Radiography was taken for each graft at 2,4,8,12 week. 12 weeks after operation, the patency of the pedicle was observed through ink infusing and histological studies were performed.Results Group A-D was better than group E obviously in the fields of patency rate, levels of IFN-γ, osteosis of bone allografts, histological examination. There was no difference of IFN-γbetween...