The Effects And Clinical Roles Of HLA-DRB1 Alleles And Anti-CCP Antibody In The Pathogenesis Of Rheumatoid Arthritis

Objective To explore the effects and clinical roles of HLA-DRBl alleles and anti-CCP antibody in the pathogenesis of rheumatoid arthritis and explore pathogenesis of rheumatoid arthritis by analyzing the correlation of HLA-DRB1 alleles and anti-CCP antibody with severe RA and the correlation of HLA-DRBl alleles with anti-CCP antibody.Methods 106 RA patients and 340 healthy blood donors were selected by random sample.Case-control method was used for study. Multiple sequence specific primer PCR(SSP-PCR) was used to amplify HLA-DRBl alleles. First, the method of low resolution SSP-PCR was used to amplify HLA-DRBl alleles; second, the method of high resolution SSP-PCR was used to amplify HLA-DRB1*O4, DRB1*10, DRB1*01 and DRB1*14 subtypes;then, allele carriage rates between RA patients and healthy controls were caculated and compared by Pearson Chi-square test or Fisher's Exact Test. When P≤0.05,the difference between two group was considered significantly in statistic,.Anti-CCP antibody in serum of RA patients was detected by enzyme-linked immunosorbent assay (ELISA).All these patients were grouped into severe RA group and non-severe RA group based on X-ray expression of joints.The correlations of HLA-DRBl alleles, anti-CCP antibody, RF and the course of disease with severity of disease were analyzed by Logistic regression. Pearson Chi-square test was used to analyze the correlation of HLA-DRBl alleles and anti-CCP antibody.SPSS11.5 was used.The level of detecting of P≤0.05 was selected.Results 1 Thirteen subtypes of HLA-DRB1 alleles were acquired by low resolution SSP-PCR. RA patients have higher frequencies of HLA-DRB1*04,*09 and *1001...