Cytogenetics And Molecular Genetics Of Chronic Myeloid Leukemia

Objective To study the cytogenetic and molecular genetic changes of chronic myeloid leukemia (CML) in blast crisis(BC) and discuss the correlation between these changes and clinical features. Simultaneously, to explore the value of fluorescence in situ hybridization (FISH) and multiplex fluorescence in situ hybridization (M-FISH) techniques in the detection of genetic changes in CML carrying variant Philadelphia translocation (vPh).Methods Bone marrow cells from 25 CML-BC cases including 7 transformed to acute lymphoid leukemia (CML-ALL) and 18 transformed to acute non-lymphoid leukemia (CML-ANLL) were cultured in direct and indirect methods. Cells were harvested and made chromosome preparations in routine. R banding was applied in karyotype analysis. At least 20 metaphase cells were analysed each case by two people. Simultaneously these cases were assayed with BCR-ABL dual color dual fusion FISH technique. 500 interphase cells were observed each case. If only one fusion signal was detected, 10 metaphase cells were observed to determine if there were derivative chromosome 9 [der(9)] deletions.