| Objective:The purpose of this research was to explore chromosomal aberrations of metaphase cells using the DP and STC on the excised tissue samples of PAs. To increase the resolution of cytogenetic analysis,DPs from 50 PAs were investigated by interphase FISH. Combining the clinical data with pathological characters,we wanted to elucidate the correlations among chromosome changes,tumor types and biologic behaviors in order to reveal the initiation and progression of pituitary adenomas in essence. Further studies would not only provide reliable evidence of the clinical diagnosis and prognosis,and also develop a novel treatment for patients with pituitary adenomas.Materials and Methods:The research was divided into two parts. In part one,we used the sample excised from the patients to make the single cell suspensions. Both the DP and STC were set up. When the fragment size did not allow both methods to be used,the DP was preferred,by which 50 samples were done,and 25 samples were done by STC. Using R-banding to analyse the karyotypes of the samples,we initially found that there were some chromosomal abnormalities in ourgroupes,and then compared the advange and disadvange of both methods. In part two,according to the results of karyotypes,we detected the abnormal number of the 7,8,11,12,X chromosomes in interphase cells of PAs by FISH,and then combined the subtype and pathology of PAs in order to discusse the relations of the chromosomes abnormalities,subtypes and biologic behaviors of PAs.Results:Our research found out that the DP of 25 tumors allowed us to identify a karyotype in 17 samples (17/25,68.0%),whereas the STC of 25 tumors yielded a karyotype in 21samples (21/25,84.0%). An abnormal clonal karyotype was observed in 11 of the 25 samples (11/25,44.0%) successfully processed by the DP,however 3 of the 25 samples yielded an abnormal clonal karyotype. The most frequently found chromosomal alterations in our study were the gains of an entire copy of chromosome X,8,7,12,5 and the loss of chromosome 9,11,13. hi particular,the loss of chromosome was commonly found in invasive PAs. hi the recurrent PAs,the chromosome 19 participated in TAS with 17 and 19. As far as numerical aberrations were concerned,interphase FISH not only confirmed the gains of chromosome X,7,8,12 in metaphase cells,but also delected the gains of chromosomes 7 and 12 which counld not be found out in metaphase cells by CC. In addition,we found that 26 samples took on abnormal karyotypes in 34 functioning PAs,but only 6 samples had abnormal karyotypes in 16 non- functioning PAs.Conclusions:Our comparison of the cytogenetic results obtained applying both the DP and STC definitely demonstrated that there were much more metaphase cells by STC than that by DP,however the chromosomal aberrations by STC decreased much more than that by DP. The discrepancy made the DP the most suitable for the cytogenetic investigation of both the benign neoplasia and other malignant tumors. We also found that there was much discrepancy of chromosome aberrations between the functioning and non-functioning PAs. We identified the consistent gains of chromosomes X,7,8,12 and loss of chromosome 11,and also confirmed that the loss of chromosome 11 had marked correlations with the invasiveness of PAs. In addition,the structrure aberrations of chromosome 19 might participate the progression of PA,and might have some correlations with the recurrent of PA. |
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