Effects Of NO-1886 On Inflammation-associated Factors In High-fat/high-sucrose/high-cholesterol Diet-fed Miniature Pigs

Objective: To observe effects of weight gain, ectopic lipid deposition, expression levels of pro-inflammation cytokines in Guangxi Bama-miniature pigs fed by high-fat/high-sucrose/high-cholesterol diet (HFSCD); and to study whether the long-term administration of the LPL activator, NO-1886, inhibit obesity, decrease weight gain, lower the expression of inflammation association cytokines, and alleviate inflammation status, thus protecting against the development of atherosclerosis and diabetes mellitus.Methods: 1) Animal preparation and treatment: Fifteen male Chinese Bama-miniature pigs were randomized into three groups with similar body weight [n=5 in the normal control diet (CD) group; n=5 in the high-fat/high-sucrose/high-cholesterol diet (HFSC diet) group; n=5 in the HFSC diet supplemented with NO-1886 (HFSC diet +NO-1886) group]. The total study period was 5 months. 2) The levels of glucose and lipid plasma parameters were studied: Blood samples for plasma parameters, including glucose, insulin, triglyceride (TG), free fatty acid (FFA), high-density lipoprotein cholesterol (HDL-C), oral glucose tolerant test (OGTT) and insulin sensitivity test, were withdrawn from the animals at the end of each month following an overnight fast. 3) The levels of plasma C-reactive protein (CRP) and plasminogen activator inhibitor-1 (PAI-1) were measured. 4) The levels of the expressions of 40 inflammation cytokines in fat tissues were determined using inflammation antibody arrays. 5) The microscope structure of liver and muscle tissues were observed with transmission electronic microscopy; the degree of lipid deposition in liver, skeletal muscle and cardiac muscle tissues were examed by oil red O stain; the infiltrations of inflammation cells in tissues were examed by HE stain; the aortic lesions were analyzed by Sudan IV stain; the vascular cell adhesion molecule-1(VCAM-1) in aortic tissues was examed by immunohistochemical staining. 6) The levels of LPL in liver, muscle and adipose tissues were measured by reverse transcription-polymerase chain reaction (RT-PCR). The levels of inflammation...