NO-1886 Improves Glucose And Lipids Metabolism In Diabetic Bama Minipigs

Objectives To observe high-fat/high-sucrose/high-cholesterol diet inducing the changes of glucose and lipid metabolism and try to establish a new animal model of the human type 2 diabetes mellitus; to investigate the effects of NO-1886 on glucose and lipid metabolism and functions of the pancreatic islet; To study the effects of NO-1886 on β-cell apoptosis and the protein expression of bcl-2/bax from the islet in Guangxi Bama minipigs.Materials and methods Animals and diets: Guangxi Bama minipigs were randomized into three groups with similar body weight: control group, high-fat /high-sucrose/high-cholesterol group, high-fat/high-sucrose/high-cholesterol with NO-1886 treated group. The animals were fed with control diet, high-fat/high-sucrose/ high-cholesterol diet and high-fat/high-sucrose/high-cholesterol diet supplemented with 1%NO-1886 for 5 months. Blood samples were withdrawn from the orbital sinus of the animals at the end of each month after fasting overnight. Blood glucose, insulin, glucagons, TG, TC, Amylin, FFA and HDL-C were determined. An oral glucose tolerance test (OGTT) was performed every month. The animals were sacrificed at the end of month 5. The pancreas tissues were dissected from each animal. The effects of NO-1886 on morphology of pancreas were analysed by HE stain, immuno-histological stain, fluorescence staining, Congo Red stain, the apoptosis of p-cell was observed by light and electron microscopy. The levels of bcl-2 and bax in islet were measured by immunohistochemistry and Western blot.Results Feeding a high-fat/high-sucrose/high-cholesterol diet to miniature pigs increased plasma levels of TG, TC, FFA, glucose, glucagon, Amylin, and decreased the...