The Research Of Biomarkers In Rats Following Mechanical Brain Injury

Objective: To study the alteration of protein expression pattern in rats serum and hippocampus after closed head injury and to find biomarkers of brain injury.Methods: Male Sprague-Dawley rats were randomly divided into sham operation group and injury groups which were subjected to Marmarou's brain injury and then subdivided into 4 h,8 h,12 h,24 h and 48 h groups according to the time elapsed after injury. The pathological study included H&E stain, toluidine blue stain. The change of protein expression pattern in serum and hippocampus were monitored with strong anion exchange chips (SAX2) and hydrophobic surface chips(H4) by surface-enhanced laser desorption ionization-time of flight-mass spectrometry.Results: 1. Clinical changes: All rats of injury groups had no skull fracture. Immediately after brain injury, 70% of rats generalized convulsions with apnea for 15 seconds. 9.17% of rats developed convulsions without apnea for 30 seconds. 14.3% of rats weakened quadruped motor function.2. Macroscopic findings: A massive and extensive subarachnoid hemorrhage could be found in all injured rats and covered the entire brain. There were no contusions in brain tissues.