Study On Influencing Rat Fracture Healing By Injection Of "Gu Kang Tai Lin"

Objective: To explore the effects of soluble multi-peptide agent, marked "Gu Kang Tai Lin", derived from bone tissue on rat bone fracture healing at different time intervals. And observe the functional changes of adjustment in pituitary to discuss the mechanisms of promoting union of fracture with "Gu Kang Tai Lin". Methods: Fifty-four adult SD rats(200 ± 20g, female) were used for this investigation. Closed fracture of radius of the rest forty-eight female rats were produced except for six rats in the blank group. Then they were divided randomly into 2 groups: the GuKangTaiLin group and the Model group. Six rats in each group were executed on the 7th, 14th,28th and 42th day after operation to observe the radiographic findings; bone mineral density (BMD) measurements; histological and morphological changes in osteotylus; the GH cells and TSH cells in pituitaries were showed by immunocytochemical method. Results: At different time intervals the radiographic findings, bone mineral density (BMD) measurements of the fractural indicated that the GuKangTaiLin group had more bony calluses than the Model group (P<0.05); And histological changes demonstrated that bone trabecula of the GuKangTaiLin group were more mature than those in the model group. At broken ends of fractured bone, a great deal of bone trabecula around the bone callus lined up in order. On the other hand, the results of statistical analysis showed: The peak amplitude of GH cells and TSH cells population of the GuKangTaLin group were obviously anterior to the...