Studies On Chemical Constituents And Bacteriostatic Activities Of Buddleja Purdomii W.W Smith

Buddleja purdomii W.W Smith, is one of Buddleja L.,which distributeas in min.county,wudu county,zhouqu county of Gansu province.It is a kind of natural plant which cannot be found in the rest of China,except for Gansu.So far no relevant medicinal record has been reported.This dissertation is to study its chemical constituents and bacteriostatic activities in vitro to explore whether it has medicinal value.Many studies on the chemical constituents and bioactivities of genus Buddleja L. have been done. Previous phytochemical investigation on Buddleja led to flavonoids, phenylpropanoids,iridoids,sesquiterpenes and saponins.But only a few work on the chemical constituents and bioactivities has been reported on Buddleja purdomii W.W Smith..In order to provide rational knowledge for the bioactivity and plant taxonomy,the chemical constituents and bacteriostatic activities in vitro of Buddleja purdomii W.W Smith.have been investigated in this dissertation.The chemical constituents of the 95% ethanolic extract of Buddleja purdomii W.W Smith.were studied and 10 compounds were isolated by chromatography on silica gel column and polyamide column.Their structures were identified by spectroscopic methods such as MS,¹H NMR ,IR and ¹³C NMR.Then 10 chemical constituents were established as aucubin (l),syringin(2),syringaresinol-O-β-D-glucopynanoside(3),acteoside(4)(2-ethyl -2-hydroxy- 3-methoxybutanoate-O-β-D-glucopynanoside)(5),6-(1,3-dihydroxypropan-2-yl)-3-(hydroxy-methyl)cyclohex-2-enol(6),6-(l,3-dihydroxypropan-2-yl)-3-(hydroxylmethyl)cyclohex-3-enol (7), cryptomeridiol(8), galactilol(9), and daucosterol(10).In this study,it has been found that Buddleja purdomii W.W Smith.is rich in phenylpropa-noid glycosides,terpenes and galactilol.Their bioactivities are expected to be further studied.The constituents of the low polarity part from Buddleja purdomii W.W Smith.were analysed by GC-MS. 66 constituents were identified and they comprised 43.96% of the total peak area.We used the K.-B method to observe its antibacterial action in vitro.The results showed the low polarity constituents could effectively inhabit Staphy loco cciaureus, Escherichia coli and Psedomonas aeruginasa.