Rapid Detection Of S-HpAg By ELISA

Helicobacter pylori (H. pylori) has been attracted more attention since it was separated from gastric mucosa and culture successfully by Warran and Marshall in 1982. . H. pylori is a worldwide epidemic bacteria, there are about half people infect H. pylori H. pylori is close with peptic ulcer., it has near relationship between H. pylori and gastric carcinoma (or Mucosa Association Lymphoid Tissue Lymphoma ) . HP can also give rise to eight other diseases: coronary heart disease colic, growth retardation and dyspepsia of childhood and so on. It was list as Grade 1 Carcinogen.Recently, there are two main ways, invade and non-invade, to diagnose the infection of HP. But the invade way needs to have a gastroscope to got living gastric mucous membrane specimen that will bring the patient many unnecessary pains. At the same time, the non-invade way has its limitation too, such as using radioactive isotope is which will pollute environment, needing special facility, and being affected by memorizing reaction of serum antibody then bring false negative or false positive resultThe detection of Hp soluble antigen could be used as a new method which is accurate, convenient, not being affected by memorizing reaction of serum antibody, more sensitive, more specific and suitable for clinical diagnosis, evaluation of eradication and follow-up of Hp as well as for detection in children and pregnant womenFour cell strains which can steadily excrete McAbs(monoclonal antibodies) were established in this study, named E4, B7, G2, G3. The filtration, preparation, purification, identify of the McAbs and work partnership which are the foundation of the clinical trial were finished. At the mean time The HP-ELISA detection kit is the necessary foundation of the study of golden colloid kit, and one inventive patent was applied based on this study and the patents acceptance number is 2005102002286...