| Aim:To explore the relationship of Spleen Zang and Spirit based on the theory of five viscera holding spirit. By observing the behavior change of the rat model of spleen deficiency syndrome,we mearsured level for c-jun gene with immunohistochemical staining and used hybridization in situ to detect expression of c-fos mRNA,SSR mRNA,OTR mRNA,then used Hematoxylin and eosin(HE) staining to observe the morphological change of neurons in rats, in order to reveal the mechanism of spleen deficiency influence on learning and memory. The study's final goal is to reflect the sense of TCM spleen in storing idea and in charge of thoughts,and provide new experimental evidence for TCM treating emotional diseases based on spleen.Methods:In this study, the spleen-asthenia rat model are produced by applying multiple factors such as abnormal diet, over strain and damage from abuse diarrhea drugs. Guipi decoction was used as a remedy to verify the availability of the model. Chaihushugan decoction was used as a positive contrast. Hematoxylin and eosin(HE) staining was used to reflect the morphological change of Neurons in rats, immunohistochemical method was employed to detect the level of c-jun gene, hybridization in situ was adopted to determine the neuropeptide receptor expression of C-FOS mRNA,SSR mRNA and OTR mRNA.Results:1 The spleen-asthenia rat model produced by applying multiple factors manifested symptoms similar to the typical case of spleen deficiency syndrome such as diarrhea, athrepsy, anepithymia, depressed, and so on at the thirth day. The diarrhea was severest at the eighth day. The group rat prevented and treated by Guipi decoction did not show manifestation of spleen deficiency syndrome, and compared with normal controls it did not show significant changes, which demonstrated that it was successful to make spleen-asthenia rat model.2 Four behavioral tests'results showed that the abilities of learning and memory in the...
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