Study Of Oat β-Glucan On Glycemia In Diabetic Rats

Objective To observe the effects of oat β -glucan on glycemia in diabetic rats induced by STZ and investigate its mechanism.Methods 1 To confirm the adequate dosage of STZ, we compared the model rate and livability of two different doses of STZ, 55mg/kg and 65mg/kg. 2 We observed the glycemic responses of oat β -glucan in diabetic rats. Twelve SD male rats were selected randomly for normal group by their weight and other 68 SD male rats were injected peritoneal with 55mg/kg STZ, and 59 became diabetic rats. They were randomly divided into five groups according to glucose level: model, low dose of oat β -glucan, medium dose, high dose and acarbose group. Normal and model group were given distilled water through tube feeding without any treatment every day. Low dose group was treated with oat β -glucan solution (0.125g/kg·bw) and medium group( 0.25g/kg·bw), high group (0.75g/kg·bw) every day. Acarbose group was treated with acarbose (0.02g/kg·bw) every day. The treat went on 30 days. We detected FBG through tail vein in the fifteenth day and thirty-first day. We killed all rats and surveyed their serum Bun, Cr, lipids, SOD, GSH-Px, MDA, GSP, insulin and C-peptide and liver hepatin, SOD, MDA. 3 The effect of oat β -glucan of weight , glucose tolerance in normal rats and blood glucose, antioxodation in diabetic rats. Forty male SD rats were randomly divided into three groups by their weight: normal group, model group and protection group. The normal group and model group were given distilled water every day. The protection group was treated with oat β -glucan( 0.25g/kg·bw) through tube feeding per day. The treatmentlasted 21 days. We weighted all rats at the end of each week. We did glucose tolerance test in the twenty-second day. First we detected FBG, then ig glucose solution( 2g/kg*bw) , measured blood glucose at 30,60,120 min. In the twenty-third day, the model and protection group were given water and oat 0 -glucan( 0.25g/kg*bw) respectively. Two hours later, they were injected peritoneal with 55mg/kg STZ. After 7 days, we detected FBG through tail vein and serum SOD, MDA.Results 1 There was no difference in the model rate between A group and B group, but the livability of A group was significantly increased(P<0.05). 2 Compared with model group, FBG of high dose group (the thirty-first day) was significantly decreased (P<0.01). TC and TG of med, high dose group were lower than model group (P<0.05). Cr of three doses of groups was significantly decreased(P<0.01) compared with model group. Compared with normal group, the activity of SOD, GSH-Px, the content of insulin, C-peptide were declined significantly, the content of MDA, GSP were raised significantly in diabetic rats. Compared with model group, the activity of SOD, GSH-Px, the content of insulin, C-peptide were increased (P<0.05) through treatment of oat P -glucan, the content of MDA, GSP were reduced significantly (P<0.05) through treatment of oat P -glucan. 3 Compared with normal group, there was no difference in FBG of 0, 60 min of protection. But FBG of 30 min was significantly lower than normal group(P<0.05). After treatment of oat 3 -glucan, the weight increase of protection group was declined significantly compared with model group. Compared with model group, FBG and content of serum MDA of protection group were reduced significantly (P<0.01), and the activity of SOD was increased significantly (P<0.01).Conclusion 1 55mg/kgofSTZ (ig) was the adequate dose of diabetic rat. 2 Oat P -glucan had remarkable hypoglycemic effect in diabetic rats induced by STZ. It could regulate TC and TG, promote the function of antioxidation, protect the function of kidney, increase the content of liver hepatin. 3 After ig Oat0 -glucan in normal rats, the glucose tolerance could be improved and the weight increase was restrained. Oat 0 -glucan had an active effect of provention in diabetic rats induced by STZ.