Effects Of Sustained PolyI:C On The Maturation Of BMDC And Microarray Analysis Of Sustained CpG Stimulated BMDC

Objective: To study the effect of sustained PolyI:C or CpG stimulation to the maturation of murine bone-marrow derived dendritic cells (BMDCs). Methods: Cell phenotypes and antigens uptaken ability were analyzed by flow cytometry. Cytokines were detected by ELISA. Antigen presenting ability was evaluated by mixed lymphocyte responses. Results: Comparing to the BMDCs of PolyI:C once group, BMDCs cosultured with PolyI:C show remarkably lower expression of MHC-II, CD86, CD80, CD40 and secretion of IL-6, IL-12p40, IL-1β, TNF-α, IFNγ and MMP9, and enganced phagocytosis ability.but theirability of stimulating allogenic T lymphcyte was impaired. In microarray study,we found that the gene expression of MHC-II, CD86, CD80,CD40, IL-12p40,.IL-6, IL-16of BMDCs in the sustained CpG group is significantly downregulated compared to those BMDCs in the CpG once group as well as BMDCs in the control group.Conclusion: These results indicate that sustained PolyI:C or CpG stimulation can suppress the maturation of DCs, which may be the reason why the adaptive immunity is low in sepsis patients.