The Engraftment Of Liver Epithelial Progenital Cells Into Different Liver-injuried Mice

We transplanted Green Fluroscence Protein labeled LEPCs into liver-injuried mice induced by different chemicals, existence and proliferation of the cells were observed. Then we made comparisons between different models to confirm a relatively good chemical-induced liver injuried animal model. We established acute or chronic liver injury in mice with CCI₄ and monocrotaline, then we transplanted EGFP labeled LEPCs into those models. The results indicated that Green fluroscence was not detected in acute and chronic liver-injuried mice induced by CCI₄, while cell clusters were found in liver-injuried mice induced by monocrotaline. The results of HE staining and Immunohistochemistry indicated that there is no significant difference between engrafted cells and host hepatocytes in morphology and function. Our experiments demonstrated that liver-injuried mice induced by monocrotaline could provide a relatively proper liver environment for the engraftment of liver stem cells.